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Mouse Sirt6-/- TNF-alpha timecourse

ABSTRACT: We completed genome-wide microarray analysis in Sirt6 knockout (ko) mouse embryonic fibroblasts (MEFs) and control wildtype (wt) MEFs. Prior to RNA extraction, MEFs were treated with TNF-alpha (10 ng/ml) for the indicated times. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Ambion Amino Allyl MessageAmp II Amplification kit. Amplified mouse Universal Reference RNA (Stratagene) was used as reference RNA for analysis. Array hybridization of mouse MEEBO arrays is as described (Hendrickson et al., PLoS ONE, 2008). Groups of assays that are related as part of a time series. Compound Based Treatment: 10 ng/ml TNF-alpha Keywords: time_series_design

ORGANISM(S): Mus musculus

PROVIDER: GSE13207 | GEO | 2009/01/09

SECONDARY ACCESSION(S): PRJNA114029

REPOSITORIES: GEO

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Mouse Sirt6-/- TNF-alpha timecourse

Project description:We completed genome-wide microarray analysis in Sirt6 knockout (ko) mouse embryonic fibroblasts (MEFs) and control wildtype (wt) MEFs. Prior to RNA extraction, MEFs were treated with TNF-alpha (10 ng/ml) for the indicated times. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Ambion Amino Allyl MessageAmp II Amplification kit. Amplified mouse Universal Reference RNA (Stratagene) was used as reference RNA for analysis. Array hybridization of mouse MEEBO arrays is as described (Hendrickson et al., PLoS ONE, 2008). Groups of assays that are related as part of a time series. Compound Based Treatment: 10 ng/ml TNF-alpha Keywords: time_series_design Computed

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Mouse Sirt6-/- tissues

Project description:We harvested liver, spleen, thymus, and skin tissues from 21 or 29 day old Sirt6-/- animals and then completed genome-wide microarray analysis. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Amino Allyl MessageAmp II Amplification kit. Amplified age-matched wild-type tissues were used as reference RNA for analysis. Array hybridization of mouse MEEBO arrays is as described (Hendrickson et al., PLoS ONE, 2008). Set of arrays that are part of repeated experiments Tissue type: Age & tissue harvested Keywords: Biological Replicate

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IKKβ binds NLRP3 providing a shortcut to inflammasome activation for rapid immune responses

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Mouse Sirt6-/- tissues

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