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Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq 2]

ABSTRACT: To investigate the effect of Hippo-YAP signaling in insterinal stromal cells and lacteal LECs, we performed bulk and single-cell RNA-sequencing by using intestinal stromal cells isolated from mice PDGFRb-specific deletion of LATS1/2

ORGANISM(S): Mus musculus

PROVIDER: GSE132092 | GEO | 2020/07/22

REPOSITORIES: GEO

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Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [RNA-seq]

Project description:To investigate the effect of Hippo-YAP signaling in insterinal stromal cells and lacteal LECs, we performed RNA-sequencing by using intestinal stromal cells isolated from mice PDGFRb-specific deletion of LATS1/2

2020-07-22 | GSE124471 | GEO

Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq]

Project description:To investigate the effect of Hippo-YAP signaling in insterinal stromal cells and lacteal LECs, we performed bulk and single-cell RNA-sequencing by using intestinal stromal cells isolated from mice PDGFRb-specific deletion of LATS1/2

2020-07-22 | GSE124487 | GEO

Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2

Project description:Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2

| PRJNA512182 | ENA

Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [RNA-seq]

Project description:Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [RNA-seq]

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Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq]

Project description:Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq]

| PRJNA512184 | ENA

Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq 2]

Project description:Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [scRNA-seq 2]

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Lats1/2 control TGF-beta directed EMT in the dorsal cranial neuroepithelium through YAP regulation

Project description:Hippo signaling, an evolutionarily conserved pathway involved in organ size control, has been implicated to play key roles in developmental processes of various tissues, but its role in craniofacial development has not been largely explored. To examine the function of the Hippo signaling kinase cascade, we inactivated Hippo components Lats1 and Lats2 in the cranial neuroepithelium of mouse embryos using a Wnt1CreSOR driver. Double conditional knock out (DCKO) of Lats1/2 resulted in neural tube and craniofacial defects. Lats1/2 DCKO mutant embryos presented a minute head with delayed and defective neural tube closure. Furthermore, neuroepithelial cell polarity and cell integrity were disrupted within the cranial neural tube in Lats1/2 DCKO mutants. Embryonic neural tube RNA-seq revealed increased TGF-beta signaling in absence of Lats1/2. Moreover, markers of epithelial-to-mesenchymal transition (EMT) were upregulated in the cranial neural tube. Notably, modulation of Hippo signaling downstream effectors Yap and Taz prevented neuroepithelial defects, aberrant EMT, and TGF-beta dysregulation caused by Lats1/2 deficiency, indicating that Lats1/2 function via canonical Hippo-Yap pathway. Together, our findings revealed important roles for Hippo signaling kinases in pre-migratory neural crest EMT and migration.

2022-09-05 | GSE182721 | GEO

The Hippo pathway kinase LATS1 scaffolds Beclin1 in autophagy regulation in hepatocellular carcinoma

Project description:Autophagy perturbation represents an emerging therapeutic strategy in cancer. The mammalian Hippo pathway is an evolutionary conserved tumor suppressive network, where the kinases LATS1/2 phosphorylate and inactivate oncogenic YAP/TAZ. Here, we demonstrated a pro-survival role of LATS1, but not LATS2, in hepatocellular carcinoma cells in response to sorafenib, a standard care of advanced HCC. Transcriptomic analysis revealed a restrictive role of LATS1 in autophagy regulation in HCC cells. Further, we found that the autophagy regulation by LATS1 was independent of its kinase activity. Instead, LATS1 stabilized autophagy core-machinery component Beclin 1 via promoting a non-canonical form of K27-linked ubiquitination, and consequently, inactive Beclin 1 self-dimer, in a E3 ligase NEDD4 dependent manner. Our study highlights a functional diversity between LATS1 and LATS2 and uncovers a scaffolding role of LATS1 in mediating a cross-talk between Hippo signalling and autophagy in HCC and therapy response.

2019-11-13 | GSE117116 | GEO

Gene expression analysis of wild type and Lats1/2-mutated uveal melanoma

Project description:Lats1/2 kinases are the core Hippo pathway kinases. In this study, we use RNAseq to analyze the gene expression profiles of adult mouse uveal tract cells and mouse uveal melanoma generated by Lats1/2 deletion induced by AAV-based Cre injection into mouse uveal tract.

2020-11-10 | GSE115181 | GEO

Expression profiling in primary embryonic fibroblasts deficient for the N-terminal truncation of Lats1

Project description:Lats1 and Lats2 are the mediators of the Hippo pathway that regulates tissue growth and proliferation. Lats1 and Lats2 kinases share 85% sequence identity in the kinase domain. However, their non-kinase regions at the N-terminus are distinct except for Lats conserved domain 1 (LCD1) and LCD2, suggesting that their N-terminal regions are important for Lats1/2-specific functions. In this study, we generated Lats1 knockout mice disrupting the N-terminal region containing LCD1 (Lats1M-NM-^TN/M-NM-^TN). We show that some Lats1M-NM-^TN/M-NM-^TN mice were born safely and grew normally. However, mouse embryonic fibroblasts (MEFs) from Lats1M-NM-^TN/M-NM-^TN mice displayed drastic defects in mitosis, showing enhanced centrosome overduplication, chromosomal misalignment, multipolar spindle formation, chromosome bridging, and cytokinesis failure. Moreover, they displayed accelerated cell cycle and cell growth bypassing a cell-cell contact inhibition like tumor cells, and exhibited anchorage independent growth. Indeed, Lats1M-NM-^TN/M-NM-^TN MEFs produced tumors in nude mice after subcutaneous injection, although the tumor growth rate was much slower than ordinary cancer cells. Furthermore, Yap, the key transcriptional co-activator in the Hippo pathway, was overexpressed and retained stable in Lats1M-NM-^TN/M-NM-^TN MEFs under high cell density, and expression of Lats2 mRNA were down-regulated. Total RNAs were extracted from MEFs under high or low cell density using miRNeasy extraction kit (Qiagen). A Dye-swapped experiment was performed by hybridizing complimentary RNA (cRNA) labeled with either Cyanine (Cy) -3 or Cy-5 (Perkin-Elmer) onto Whole Mouse Genome Oligo Microarray (G4122F; Agilent Technologies). Co-hybridizations were performed and data from Cy3 or Cy5 channels were analyzed as normalized signal intensities rather than as log ratios corresponding to each array.

2012-12-17 | E-GEOD-37871 | biostudies-arrayexpress

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