Hypoxic response in wild type and HIF-1alpha null hepatoctyes

ABSTRACT: The primary aim of this study was to evaluate the changes in hepatocyte gene expression under short-term hypoxic conditions in wild type and HIF-1a null cultures. To this end, hypoxia treated cultures were subjected to incubation with 1% O2/5% CO2/94% N2 at 37° C for eight hours prior to RNA isolation. Duplicate normoxic controls were established from separate animals wherein cultures were untreated and treated with Adbgal. Biological triplicates of wild type and HIF-1a null cultures were placed under hypoxic conditions and subsequently processed for microarray analysis. A total of 10 microarray hybridizations were performed. Keywords: repeat sample

ORGANISM(S): Mus musculus

PROVIDER: GSE1321 | GEO | 2006/01/28

SECONDARY ACCESSION(S): PRJNA89895

REPOSITORIES: GEO