Genomics

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Evolution of Escherichia coli to 42 °C and Subsequent Genetic Engineering Reveals Adaptive Mechanisms and Novel Mutations


ABSTRACT: mRNA sequencing was performed on E. coli evolved at 42C on M9 Minimal Media with Glucose Overall design: Growth of evolved E. coli was characterized under the same conditions as the evolution and compared to the wild type parent strain

INSTRUMENT(S): Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655)

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655  

SUBMITTER: Anand Sastry  

PROVIDER: GSE132442 | GEO | 2019-06-11

REPOSITORIES: GEO

Dataset's files

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GSE132442_fpkm_42c.csv.gz Csv
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Publications

Evolution of Escherichia coli to 42 °C and subsequent genetic engineering reveals adaptive mechanisms and novel mutations.

Sandberg Troy E TE   Pedersen Margit M   LaCroix Ryan A RA   Ebrahim Ali A   Bonde Mads M   Herrgard Markus J MJ   Palsson Bernhard O BO   Sommer Morten M   Feist Adam M AM  

Molecular biology and evolution 20140710 10


Adaptive laboratory evolution (ALE) has emerged as a valuable method by which to investigate microbial adaptation to a desired environment. Here, we performed ALE to 42 °C of ten parallel populations of Escherichia coli K-12 MG1655 grown in glucose minimal media. Tightly controlled experimental conditions allowed selection based on exponential-phase growth rate, yielding strains that uniformly converged toward a similar phenotype along distinct genetic paths. Adapted strains possessed as few as  ...[more]

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