Transcriptomics,Genomics

Dataset Information

28

Gene expression profiles in rat lung after inhalation exposure to C60 fullerene or ultrafine NiO particles


ABSTRACT: Gene expression profiling of the rat lung after whole-body inhalation exposure to C60 fullerene and ultrafine nickel oxide (Uf-NiO) particles as a positive control were employed to gain insights into these molecular events. Overall design: Groups of 9-week-old male Wistar rats (n = 5 per group/time point) were exposed to clean air (negative control), C60 fullerene or Uf-NiO (positive control) suspension in a whole-body exposure chamber. Rats were exposed to aerosol for 6 hours a day, for 4 weeks (5 days a week). Standard chow diet was provided ad libitum during non-exposure hours. After exposure period for 4 weeks, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days and 1month (33 days) post-exposure. Lungs of anesthetized rats were perfused with physiological saline, excised, and used for C60 fullerene or nickel burdens in lungs (n = 4, left lungs), TEM analysis (n = 1, whole lungs) and microarray analysis (n = 4, right lungs).

INSTRUMENT(S): Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version)

SUBMITTER: Katsuhide Fujita  

PROVIDER: GSE13249 | GEO | 2008-10-23

SECONDARY ACCESSION(S): PRJNA109529

REPOSITORIES: GEO

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Publications


Concern over the influence of nanoparticles on human health has risen due to advances in the development of nanotechnology. We are interested in the influence of nanoparticles on the pulmonary system at a molecular level. In this study, gene expression profiling of the rat lung after whole-body inhalation exposure to C(60) fullerene (0.12mg/m(3); 4.1x10(4) particles/cm(3), 96nm diameter) and ultrafine nickel oxide (Uf-NiO) particles (0.2mg/m(3); 9.2x10(4) particles/cm(3), 59nm diameter) as a pos  ...[more]

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