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Splenic CD8+ and CD8- DCs

ABSTRACT: Analysis of gene expressions in mouse splenic dendritic cells (DCs). DCs were purified into two subsets, CD8-positive and -negative ones. DCs were expanded in vivo by injecting Flt3L-producing tumors into the backs of C57BL/6 mice. Overall design: To expand DCs in vivo, Flt3L-producing B16 melanoma cells were injected to the backs of mice. Then, 10-12 days later, splenic DCs were enriched by MACS and purified into CD3-B220-CD8a+CD11c+ and CD3-B220-CD8a-CD11c+ cells by FACS cell sorter.

REANALYSED by: E-GEOD-45704GSE119128

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus

SUBMITTER: Hiroaki Hemmi

PROVIDER: GSE13250 | GEO |

SECONDARY ACCESSION(S): PRJNA109531

REPOSITORIES: GEO

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