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Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

ABSTRACT: We utilized the eyeless sea anemone, Nematostella vectensis, to quantify gene expression differences between different colors of light (red, green, blue) and in constant darkness through comparisons of 96 transcriptomes

ORGANISM(S): Nematostella vectensis

PROVIDER: GSE132526 | GEO | 2019/06/12

REPOSITORIES: GEO

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Post transcriptional modifications of miRNAs in the cnidarian Nematostella vectensis

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2017-08-15 | GSE94526 | GEO

The chromatin-regulating CoREST complex is animal specific and essential for development in the cnidarian Nematostella vectensis

Project description:Using the sea anemone Nematostella vectensis as a model for early branching metazoans, we show that CoREST is an animal-specific protein that assembles a conserved histone-modifying complex including Lsd1 and HDAC1/2. We further show that the Nematostella complex is similar in composition to vertebrates.

2022-08-08 | PXD033068 | Pride

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria).

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Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

Project description:Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

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RNA-seq of NvNcol3::mOrange2 positive and NvNcol3::mOrange2 negative cells from Nematostella vectensis

Project description:NvNcol3::mOrange2 is a stable transgenic line that labels cnidocytes(stinging cells) of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvNcol3::mOrange2 positive and negative cells were enriched by FACS.

2021-07-01 | E-MTAB-9556 | biostudies-arrayexpress

RNA-seq of NvElav1::mOrange positive and NvElav1::mOrange negative cells from Nematostella vectensis

Project description:NvElav1::mOrange is a stable transgenic line that labels a large fraction of the nervous system of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvElav1::mOrange positive cells were enriched by FACS.

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Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian.

Project description: Not available

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MAPK signaling promotes embryonic neural induction and expression of embryonic patterning genes in Nematostella vectensis

Project description:While FGF mediated MEK/ERK signaling is required for apical tuft formation and metamorphosis in the sea anemone Nematostella vectensis (Rentzsch et al, 2008), nothing is known about the role of MEK/ERK signaling in inducing germ layers and cell types during early developmental stages. We therefore performed a genome wide expression array on UO126 (MEK inhibitor) treated blastula stages compared to DMSO treated control embryos and identified genes potentially involved in neurogenesis, germ layer specification and axial patterning.We performed transcriptional profiling of Nematostella vectensis blastula stages (24 hours post fertilisation @ 17C) using a custom made whole genome array (4x72K - A-MEXP-2380). DMSO treated wild-type embryos were compared to U0126 (MEK Inhibitor) treated embryos at the blastula stage.

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Cnidarian cell type diversity revealed by whole-organism single-cell RNA-seq analysis

Project description:We performed single-cell transcriptome analysis (using MARS-seq) in the cnidarian Nematostella vectensis. Additionally we performed bulk ATAC-seq experiments on wild-type specimens, as well as ELAV::mOrange reporter transgenic lines (separating ELAV+ and ELAV- cell populations, representing neurons and non-neurons, respectively).

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Deorphanization of neuropeptide GPCRs in Nematostella vectensis

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