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Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 1]

ABSTRACT: Skin samples from wild type or HIRA knockout mouse embryos were dissociated to give cellular suspensions, then single cell RNA sequencing was performed using the 10X platform. Method: Cellular suspensions of skin cells from wild type and HIRA knockout mice were prepared, then sequencing was performed using the Chromium 10X system. Data were then processed using Cellranger version 2.2.0.

ORGANISM(S): Mus musculus

PROVIDER: GSE132543 | GEO | 2025/06/11

REPOSITORIES: GEO

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Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 2]

Project description:Skin samples from wild type or HIRA knockout mouse embryos were dissociated to give cellular suspensions, then single cell RNA sequencing was performed using the 10X platform. Method: Cellular suspensions of skin cells from wild type and HIRA knockout mice were prepared, then sequencing was performed using the Chromium 10X system. Data were then processed using Cellranger version 2.2.0.

2025-06-11 | GSE132544 | GEO

Effect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart

Project description:We performed gene expression profiling by microarray using RNA extracted from healthy free wall left ventricle tissues from control and Hira cardiomyocyte-specific conditional knockout mice at 6 weeks of age. Hira is a histone chaperone responsible for replication-independent incorporation of histone variant H3.3 at actively transcribed regions. Conditional knockout of Hira in cardiomyocytes resulted in impaired cardiac function, cardiomyocyte degeneration and focal replacement fibrosis. These results illustrate the role of Hira in controlling the cardiac gene program. 4 animals per group (control and Hira conditional knockout) hybridized in triplicate. RNA was extracted from healthy free wall left ventricle.

2016-04-06 | E-GEOD-71833 | biostudies-arrayexpress

Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout

Project description:Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout

| PRJNA548326 | ENA

Effect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart

2016-04-06 | GSE71833 | GEO

Chromatin assembly factor HIRA is essential for the maintenance of hematopoiesis

Project description:HIRA is a histone chaperone that deposits the histone variant H3.3 in transcriptionally active genes. HIRA is essential for mouse development, as the standard knockout (KO) results in early embryonic death. However, the role of HIRA in hematopoiesis is poorly understood. We investigated the effect of Hira KO on hematopoiesis using Vav-Cre Loxp system. We show that Hira KO dramatically reduces bone marrow LSK cells, resulting in anemia, thrombopenia and severe, combined immunodeficiency. To investigate the molecular mechanisms, RNA-seq and ATAC-Seq were performed using LSK cells isolated from WT and Hira conditional KO mice.

2020-06-30 | GSE125154 | GEO

Effect of HIRA deletion on gene expression in mouse skeletal muscle at 6 weeks of age

Project description:We performed gene expression profiling by microarray using RNA extracted from the tibialis anterior of control and Hira myofiber-specific conditional knockout mice at 6 weeks of age. Hira is a histone chaperone responsible for replication-independent incorporation of histone variant H3.3 at actively transcribed loci. Conditional knockout of Hira in myofibers caused hypertrophy, degeneration, and increased the percentage of type I fibers. These results illustrate the physiological consequences of disrupting Hira-mediated chromatin assembly in myofibers.

2017-06-26 | GSE97359 | GEO

Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 2]

Project description:Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 2]

| PRJNA548333 | ENA

Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 1]

Project description:Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 1]

| PRJNA548334 | ENA

Single cell transcriptomics of Ewing Sarcoma cell lines

Project description:We report single cell gene expression profiles for Ewing Sarcoma cell lines CHLA9, CHLA10, and TC71. Cell lines were harvested and prepared as single cell suspensions with high viability. Library prep was performed with the 10X Genomics v3.1 3' kit. All samples were sequenced on the illumina HiSeq 3000 with a 28+8+100 configuration. CHLA9 and CHLA10 libraries were re-sequenced on the Illumina NovaSeq with a 28+8+91 configuration. Counts were processed with cellranger v3.1.0. Both the raw data and the cellranger output are provded.

2020-04-11 | GSE146221 | GEO

Inactivation of histone chaperone HIRA unmasks a link between normal embryonic development of melanoblasts and maintenance of adult melanocyte stem cells (ATAC-Seq)

Project description:Evidence indicates that the integrity of in utero embryonic development can influence late life healthy or unhealthy aging. However, specific links between embryonic development and late life aging are not well defined. Histone chaperone HIRA is thought to play a role in both early development and aging. Here, we explore the relationship between HIRA’s roles in development and aging by comparing lineage-specific constitutive and conditional Hira knock out in the murine pigmentary system, with constitutive knock out initiated during embryogenesis and conditional knock out in young adults. Embryonic Hira knockout leads to reduced melanoblast numbers during embryogenesis but wild type numbers of differentiated melanocytes at birth, normally functioning juvenile and young adult melanocyte stem cells (McSCs), and only a very mildly hypopigmented first hair coat. However, on closer analysis of these mice, Hira knockout melanocytic cells of newborn mice exhibit molecular markers characteristic of cell aging and proliferative deficits. As they age, mice with Hira knock out initiated during embryogenesis display marked defects in McSC maintenance and premature hair graying. Importantly, these defects are only observed when HIRA is inactivated during embryogenesis, but not in young adults. This genetic model shows that HIRA function during early development lays a foundation for subsequent maintenance of adult tissue specific stem cells during aging.

2025-06-11 | GSE267431 | GEO

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