Dataset Information

Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites

ABSTRACT: Kinetoplastids are a group of parasite species, several of which cause important diseases in human and livestock. Nearly all of these pathogenic species are transmitted by insect vectors, in which the parasites undergo a specific developmental program. One shared event undergone by multiple species is adherence to insect tissue. This adhesion occurs by means of a hemidesmosome-like structure that is thus far uncharacterized. We have used the monoxenous parasite Crithidia fasciculata, which exclusively infects mosquitoes, to study this process of parasite adhesion in the insect. We have transcriptionally profiled adherent and swimming forms of the parasite that have been generated in vitro, and compared these profiles to the adhesive form in the mosquito. Using a dual-RNAseq approach, we have also identified several genes that are differentially regulated in infected versus uninfected mosquitoes, including several immune genes. This indicates that the mosquito is responding to the presence of the parasites.

ORGANISM(S): Crithidia fasciculata Aedes aegypti

PROVIDER: GSE132641 | GEO | 2019/06/26

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. Results: We identified 4047 genes expressed in hemocytes, using An. gambiae genome-wide microarrays. While 279 transcripts were significantly enriched in hemocytes relative to whole adult female mosquitoes, 959 transcripts exhibited immune challenge-related regulation. The global transcriptomic responses of hemocytes to challenge with different species of bacteria and/or different stages of malaria parasite infection revealed discrete, minimally overlapping, pathogen-specific signatures of infection-responsive gene expression; 105 of these represented putative immunity-related genes including anti-Plasmodium factors. Of particular interest was the specific co-regulation of various members of the Imd and JNK immune signaling pathways during malaria parasite invasion of the mosquito midgut epithelium. Conclusion: Our genome-wide transcriptomic analysis of adult mosquito hemocytes reveals pathogen-specific signatures of gene regulation and identifies several novel candidate genes for future functional studies. In order to identify hemocyte-specific and immune-responsive transcripts, we first compared transcripts expressed in hemocytes from one day old sugar-fed mosquitoes to transcripts detected in whole mosquitoes of the same age and feeding status. This resulted in identification of the hemocyte-enriched transcriptome. We then compared hemocytes from 1 day old mosquitoes, 1 hour after immune challenge with heat-killed Escherichia coli or Micrococcus luteus, to control female mosquitoes injected with sterile PBS to determine the bacteria challenge responsive transcriptomes. We used heat-killed bacteria in these assays, because our primary interest was in identifying the bacterial responsive transcriptome and to avoid the potentially confounding effects of altered gene expression due to the lethal effects of a systemic infection associated with injection of living bacteria. Lastly, we compared hemocytes from mosquitoes at 24 hours and 19 days after ingestion of a blood meal infected with Plasmodium berghei to mosquitoes of the same age fed a non-infected blood meal to determine the ookinete and sporozoite infection responsive transcriptomes, respectively. This design resulted in a total of five experimental treatments. The following samples are not included in this submission: Hemo E coli vs. hemo unchallenged A Hemo E coli vs. hemo unchallenged B Hemo m luteus vs. hemo unchallenged A Hemo m luteus vs. hemo unchallenged B

Dataset Information

Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure