Genomics

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Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq


ABSTRACT: We develop a method Re-Cappable-seq for determining eukaryotic transcription start sites derived from all RNA polymerases at nucleotide resolution. In particular, this method identifies the Pol-I and Pol-III TSSs, which are missing by CAGE. Applied to human A549 cell line, our method results in the identification of 33,468 and 5,269 high confidence Pol-II and non-Pol-II TSS respectively. Re-Cappable-seq identifies Pol-II TSS with higher specificity than CAGE.

ORGANISM(S): Homo sapiens

PROVIDER: GSE132660 | GEO | 2019/06/14

REPOSITORIES: GEO

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