Project description:Single cell sequencing of Tomato+ cells in hindlimb muscles from Prrx1-CreERT Tomato mice with the treatment of Tamoxifen for 3 days
Project description:We had one goal to perform this study: Analysis of distal part of the auricle from three Prrx1-CreERT;Bmpr1af/f and age- and gender-matched control mice.
Project description:Single cell sequencing of Tomato+ cells in Inguinal White Fat from Prrx1-CreERT Tomato mice with the treatment of Tamoxifen for 3 days
Project description:Primary human adipose stromal cells (hASCs) from rs4684847 CC risk allele carriers were cultured and induced to differentiate into adipocytes, and simultaneously transfected for 72h with non-targeting siRNA or siRNA targeting PRRX1 (n=10) or both PRRX1 and PPARG (subset of the subjects, n=4).
Project description:During neonatal development, skeletal muscle grows dramatically by myonuclei accretion to existing fibers and hypertophic growth of fibers with protein synthesis. To understand molecular mechanism underlying neonatal muscle growth, we used microarray to profile the global program of gene expressions especially involved in myoblast fusion, migration, and muscle fiber growth by itself. Mouse hindlimb muscles at postnatal day (P) 2, 7, and 14 were isolated and used for RNA extraction and hybridyzation on microarrays. We used three biological replicates for each time point.