Direct RNA sequencing enables single-nucleotide m6A detection in endogenous transcript isoforms

ABSTRACT: We report the direct RNA sequencing of HEK293 and a primary human mammary epithelial cell (HMEC) line using Oxford Nanopore based sequencing. Using this data, we built an algorithm to detect m6A modifications within the DRACH motif context. Evaluation of m6A sites was carried out with HEK METTL3 knockdown and HMEC ALKBH5 over expression cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE132971 | GEO | 2020/01/14

REPOSITORIES: GEO