Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BR-body mutant mRNA half-life profiling.

ABSTRACT: BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. JS38 (wild type) was compared to JS221 (lacking the intrinsically disordered CTD and unable to form BR-bodies), JS233 (unable to recruit degradosome components into BR-bodies), and JS299 (active site mutant of RNase E).

ORGANISM(S): Caulobacter vibrioides NA1000

PROVIDER: GSE133532 | GEO | 2019/06/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

BR-body mutant mRNA half-life profiling.

Project description:BR-body mutant mRNA half-life profiling.

| PRJNA551694 | ENA

Global RNA half lives in cyanobacteria

Project description:We calculated global RNA half lives for all genes in the cyanobacteria Synechococcus sp. strain PCC 7002. Samples from three replicates of the WT strain were taken before and following the addition of the antibiotic rifampicin to stop nascent transcription. RNA spike-ins were added for normalization and using the number of RNA-sequencing reads we were able to calculate half lives for genes, transcripts, and for each position on the genome.

2019-01-01 | GSE109174 | GEO

WT and RNE∆IDR mRNA half-life profiling in Sinorhizobium meliloti.

Project description:WT and RNE∆IDR strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Wild type(WT) was compared to RNE∆IDR strain.

2023-12-25 | GSE251703 | GEO

WT, ∆rhlB and ∆rppH mRNA half-life profiling.

Project description:WT, ∆rhlB and ∆rppH strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Wild type(WT) was compared to ∆rhlB (deleted RNA helicase RhlB gene) and ∆rppH (deleted RppH gene) strains

2023-04-03 | GSE228592 | GEO

Cell cycle mRNA half-life profiling.

Project description:mRNA half-life profiling in the bacterium Caulobacter crescentus was performed in synchronized cells collected from different stages of the cell cycle including swarmer cells, stalk cells (45 min post synchrony), and predivisional cells (90 min post synchrony). For each cell population, transcription was disrupted by the antibiotic rifampicin, and RNA samples were collected at different time points to measure the mRNA half-lives.

2023-04-03 | GSE228593 | GEO

Caulobacter crescentus mRNA half-lives by rifampicin transcription shutoff

Project description:Caulobacter crescentus mRNA half-lives by rifampicin transcription shutoff

| PRJNA661297 | ENA

C. crescentus BR-body enrichment

Project description:Enriched BR-body samples (JS299) samples and negative control samples (JS221) from a strain lacking BR-bodies. BR-body enrichment was performed by differential centrifugation as in 10.1016/j.xpro.2020.100205. Each sample was collected in three biological replicates.

2022-12-13 | MSV000090894 | MassIVE

Transcription profiling of mRNA decay in E. Coli degradosome mutants and their parental strains following transcriptional arrest with rifampicin

Project description:mRNA decay in E. Coli degradosome mutants and their parental strains following transcriptional arrest with rifampicin.

2005-11-01 | E-SMDB-2681 | biostudies-arrayexpress

Genome-wide analysis of the BR response in msg2 and axr2

Project description:Although a wide range of interactions between BRs and auxin have been recognized, knowledge about the direct molecular mechanism of interaction between them in specific physiological processes is very limited. In this study we found that auxin resisitent mutant msg2/iaa19 and arf7 were also resisitant to the BR effect on morphogenensis of dark-grown Arabidosis seedlings. Moreover, BR signaling transcription factor BZR1 can directly bind to promoter regions of IAA19 and ARF7. Microarray analysis revealed that a number of gene transcripts showed reduced BR response in msg2 and the control mutant axr2, suggesting the crucial role of IAA19 in mediating BR effects. Taken together, our results suggested that BRs regulate morphogenesis of dark-grown seedling by employing auxin signaling components IAA19 and ARF7.

2012-12-16 | GSE33412 | GEO

Hematopoietic Transcriptional Comparison of HSC from B10.BR/JRep and B10.BR/J (drifted) mice

Project description:Divergence has occured between the B10.BR-H2k H2-T18a/SgSnJJrep and B10.BR-H2k H2-T18a/SgSnJ (drifted) mouse strains, resulting in altered antigenic recognition and differential bone marrow engraftment capability. The microarray data demonstrate that the transcriptional profile of genes associated with hematopoiesis differs between lineage negative (as a marker for hematopoietic stem cells) bone marrow cells isolated from the B10.BR-H2k H2-T18a/SgSnJJrep and B10.BR-H2k H2-T18a/SgSnJ (drifted) mouse strains.

2009-09-16 | GSE17471 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data