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Plasma circulating miRNA profiling in polymicrobial sepsis by high throughput sequencing

ABSTRACT: Aim: To explore the potentially important role of circulating miRNAs in systemic inflammation during sepsis. Method: 8 to 10-week old C57BL/6 mice underwent cecal ligation and puncture (CLP) or laparotomy (Sham). Plasma RNA was isolated using Trizol LS reagent 24h post-surgery. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform.

ORGANISM(S): Mus musculus

PROVIDER: GSE133733 | GEO | 2020/04/12

REPOSITORIES: GEO

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