Genome-wide maps of chromatin state in A375 cells or in normal or USP7 knockdown A375 cells .

ABSTRACT: ChIP-seq experiments were performed in normal or USP7 knockdown A375 cells with specific antibodies against USP7, EZH2, H3K27me3 and H2BK120ub1. Consistent with our expectations, the analysis of ChIP-seq data showed that USP7 loss-of-function led to obviously reduction in USP7, EZH2 and H3K27me3 around the USP7-specific peaks, but an upregulation in H2BK120ub1. This study gave us a new understanding that USP7 is required for EZH2 loading and H3K27 trimethylation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE133834 | GEO | 2020/05/01

REPOSITORIES: GEO