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MiRNA expression in Drosophila

ABSTRACT: miRNA expression in adult flies infected with the Gram-positive bacteria Micrococcus luteus or the fungi Beauveria bassiana.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE133862 | GEO | 2022/07/04

REPOSITORIES: GEO

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Expression data from drosophila melanogaster

Project description:Space travel presents unlimited opportunities for exploration and discovery, but requires a more complete understanding of the immunological consequences of long-term exposure to the conditions of spaceflight. To understand these consequences better and to contribute to design of effective countermeasures, we used the Drosophila model to compare innate immune responses to bacteria and fungi in flies that were either raised on earth or in outer space aboard the NASA Space Shuttle Discovery (STS-121). Microarrays were used to characterize changes in gene expression that occur in response to infection by bacteria and fungus in drosophila that were either hatched and raised in outer space (microgravity) or on earth (normal gravity). Whole Oregon R strain drosophila melanogaster fruit flies either raised on earth or in space that were (1) uninfected, (2) infected with bacteria (Escherichia coli), or (3) infected with fungus (Beauveria bassiana) were used for RNA extraction and hybridization on Affymetrix microarrays.

2013-12-11 | E-GEOD-53196 | biostudies-arrayexpress

Transcriptomic profile of the Beauveria bassiana infection to the Galleria mellonella

Project description:The ascomycete fungus Beauveria bassiana is a pathogen of hundreds of insect species and is commercially produced as an environmentally friendly mycoinsecticide. Genome-wide insight into the infection of the fungi is critical for genetic improvement of fungal insecticides but has been poorly explored. We constructed three transcriptomes of Beauveria bassiana at 24, 48 and 72 hours post treatment of infection (BbI) and of control (Bbc).

2017-04-18 | GSE97879 | GEO

Gene expression in Drosophila melanogaster in response to bacterial infection

Project description:The goal of this study was to generate a high-quality dataset of gene expression in Drosophila melanogaster 3 hours after infection with Gram-positive (M luteus) or Gram-negative (E coli) bacteria.

2023-10-31 | GSE216042 | GEO

Global Analysis of the Transcriptional Response of Whitefly to Entomopathogenic Fungi Beauveria bassiana Reveals New Insights on Host-Pathogen Interactions

2013-11-24 | GSE38108 | GEO

Transcriptomic analysis of LaeA and LaeB deletion strains in fungus Beauveria bassiana

Project description:Transcriptomic analysis of LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana Examination of differential gene expressions by Beauveria bassiana wild type, LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana

2013-12-05 | E-GEOD-52995 | biostudies-arrayexpress

Expression of immune-inducible genes in Drosophila melanogaster whole fly samples upon Micrococcus luteus infection for 24h

Project description:Transcriptome profiling of immune-responsive genes in Drosophila melanogaster during Gram-positive bacterial infection was performed. The most highly induced gene by Micrococcus luteus infection was a novel gene CG44404, named Induced by Infection (IBIN). In the Flybase release FB2019_4 (22.8.2019) IBIN is annotated as coding for a short peptide. In the transcriptome analysis, fourteen immune-inducible long non-coding RNA (lncRNA) genes (Fold Change > 3) were also found.

2019-01-01 | GSE120387 | GEO

Global Analysis of the Transcriptional Response of Whitefly to Entomopathogenic Fungi Beauveria bassiana Reveals New Insights on Host-Pathogen Interactions

Project description:We investigated the transcriptional response of invasive Mediterranean (MED) species of the whitefly B. tabaci complex (commonly referred to as Q biotype) to entomopathogenic fungi Beauveria bassiana using Illumina sequencing technology. Nearly 1,000 of control whiteflies, 48h fungal-induced whiteflies and 72h fungal-induced whiteflies were collected, respectively.

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lncRNA-CR46018 as a positive regulator controls Drosophila Toll immune response by interacting with Dif/Dorsal  

Project description:The Toll signaling pathway is highly conserved from insects to mammals. Drosophila is a model species that is commonly used to study innate immunity. Although many studies have assessed protein-coding genes that regulate the Toll pathway, it is unclear whether long noncoding RNAs (lncRNAs) play regulatory roles in the Toll pathway. Here, we evaluated the expression of the lncRNA CR46018 in Drosophila. Our results showed that this lncRNA was significantly overexpressed after infection of Drosophila with Micrococcus luteus. A CR46018-overexpressing Drosophila strain was then constructed; we expected that CR46018 overexpression would enhance the expression of various antimicrobial peptides downstream of the Toll pathway, regardless of infection with M. luteus. RNA-seq analysis of CR46018-overexpressing Drosophila after infection with M. luteus showed that upregulated genes were mainly enriched in Toll and Imd signaling pathways. Moreover, bioinformatics predictions and RNA-immunoprecipitation experiments showed that CR46018 interacted with the transcription factors Dif and Dorsal to enhance the Toll pathway. During gram-positive bacterial infection, flies overexpressing CR46018 showed favorable survival compared with flies in the control group. Based on these findings, we renamed CR46018 as lncRNA-RDIP. Overall, our current work not only reveals a new immune regulatory factor, lncRNA-RDIP, and explores its potential regulatory model, but also provides a new perspective for the effect of immune disorders on the survival of Drosophila melanogaster.

2021-05-20 | GSE174684 | GEO

Dynamic miRNA-mRNA regulations are essential for maintaining Drosophila immune homeostasis during Micrococcus luteus infection

Project description:Pathogen bacteria infections can lead to dynamic changes of microRNA (miRNA) and mRNA expression profiles, which may control synergistically the outcome of immune responses. To reveal the role of dynamic miRNA-mRNA regulation in Drosophila innate immune responses, we have detailedly analyzed the paired miRNA and mRNA expression profiles at three time points during Drosophila adult males with Micrococcus luteus (M. luteus) infection using RNA- and small RNA-seq data. Our results demonstrate that differentially expressed miRNAs and mRNAs represent extensively dynamic changes over three time points during Drosophila with M. luteus infection. The pathway enrichment analysis indicates that differentially expressed genes are involved in diverse signaling pathways, including Toll and Imd as well as orther signaling pathways at three time points during Drosophila with M. luteus infection. Remarkably, the dynamic change of miRNA expression is delayed by compared to mRNA expression change over three time points, implying that the "time" parameter should be considered when the function of miRNA/mRNA is further studied. In particular, the dynamic miRNA-mRNA regulatory networks have shown that miRNAs may synergistically regulate gene expressions of different signaling pathways to promote or inhibit innate immune responses and maintain homeostasis in Drosophila, and some new regulators involved in Drosophila innate immune response have been identified. Our findings strongly suggest that miRNA regulation is a key mechanism involved in fine-tuning cooperatively gene expressions of diverse signaling pathways to maintain innate immune response and homeostasis in Drosophila. Taken together, the present study reveals a novel role of dynamic miRNA-mRNA regulation in immune response to bacteria infection, and provides a new insight into the underlying molecular regulatory mechanism of Drosophila innate immune responses.

2021-05-04 | GSE173733 | GEO

Transcription differences of Micrococcus luteus at late-exponential and stationary phase

Project description:Investigation of transcriptomic changes in M.luteus at 12hrs and 24hrs. Differences in fatty acid profiles of M. luteus at exponential and stationary phase is attributed to transcriptional changes of branched amino acid biosynthesis and degradation genes. This study is described by Pereira, J.H., E.B. Goh, J.D. Keasling, H.R. Beller and P.A. Adams in Crystal structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus, which has been submitted to Acta Crystallographica Section D A 6 microarray study using total RNA recovered from six separate control cultures of Micrococcus luteus NCTC2665 strain with 3 harvested after 12hrs of growth and the other 3 after 24hrs of growth. Each chip measures the expression level of 2,374 ORF based on the draft genome sequence of Micrococcus luteus with ten 60-mer probe pairs (PM/MM) per gene, with 3-fold technical redundancy.

2012-09-24 | E-GEOD-38040 | biostudies-arrayexpress

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