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Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice

ABSTRACT: This study used 10X Genomics, single-cell RNA-sequencing to examine the heterogeneity of liver non-parenchymal cell types present in the normal and CCl4-mediated fibrotic mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE134037 | GEO | 2021/06/06

REPOSITORIES: GEO

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Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice

Project description:Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice

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Single cell RNA sequencing of primary murine hepatic stellate cells from healthy versus CCl4-treated mice.

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Carbon tetrachloride (CCl4) induced liver fibrosis

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RNA-sequence data of hepatic stellate cells and liver sinusoidal endothelial cells from control and CCl4-treated mice

Project description:We utilized RNA-sequencing strategy to explore the characterstics of gene expression profile of LSECs during CCl4-fibrosis, and to compare the activation feature between fibrotic-LSECs and fibrotic-HSCs which are the most acknowledged gatekeepers holding the key to liver fibrogenesis.

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MicroRNA expression profiling of CCl4-induced liver fibrosis in Mus musculus

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Single-cell transcriptomic analysis reveals hepatic stellate cell activation roadmap and myofibroblast origin during liver fibrosis

Project description:Purpose: analyze the transcriptomic differences in liver of CCL4 and BDL mouse model Methods: Fresh isolated HSC suspensions of CCL4 and BDL were loaded on the 10x Genomics Chromium Single Cell Platform using the Chromium Single Cell 3’ GEM Library & Gel Bead Kit v3 Results: We revealed the HSC activation roadmap and portal fibroblasts are major contributor to myofbroblast in BDL model.

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Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

Project description:The cellular source for hepatocyte transplantation and liver regeneration in chronically hepatic diseases is a fundamental topic in liver biology. A successful therapeutic approach based on a benificial stem/progenitor cells would involve replacing damaged cells or restoring homeostasis to the areas that underlie the fibrotic response. This study aims to isolate and culture hepatocyte and non-parenchymal cells derived liver progenitor-like cells (HepLPCs and NPC-LPCs) to identify a beneficial cell sources for cell-based therapy against chronic liver injury. Two types of lineage tracing models including tdTomato mice with AAV8-TBG-Cre and AlbCreERT/R26GFP were purified for hepatocytes and non-parenchymal cells respectively, and cultured for transition to progenitor-like cells (LPCs) in TEM. The differences between them were clarified by RNA sequencing, suggesting that HepLPCs retaining basal levels of hepatic function and transcription factors (TFs), including Foxa3. Organoids and 3D hepatic spheroids culturing systems were used for further analysis of bile duct and hepatic functioning diversity. When transplanting in mice with CCL4 treatment, HepLPCs displayed the characteristics of TFs regulatory reactivation and physiological balance restoration, resulting in a significant improvement in liver fibrosis compared with NPC-LPCs, which are crucial for liver regeneration in the treatment of patients with a broad range of hepatic fibrosis.

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