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Expression data from wild-type and TIF1a-knockout mouse macrophages stimulated with or without mouse IL-4(20ng/mL) for 6 h.

ABSTRACT: We conducted gene expression profile analysis by using wild-type and TIF1a-knockout peritoneal macrophage.Analysis revealed the most prominent different expression of genes altered in macrophages upon IL-4 stimulation were associated with marker genes of alternative macrophage.

ORGANISM(S): Mus musculus

PROVIDER: GSE134158 | GEO | 2019/09/06

REPOSITORIES: GEO

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Expression data from wild-type peritoneal macrophages (termed WTPM) non-treated (NT) or stimulated for 6 h with mouse IL-4.

Project description:We conducted gene expression profile analysis by using wild-type peritoneal macrophages(termed WTPM).Scatter diagram revealed the most prominent different expression of genes altered in macrophages upon IL-4 stimulation were associated with M2 macrophage and Trims genes.

2019-09-06 | GSE116588 | GEO

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours.

2012-09-29 | GSE35436 | GEO

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

2012-09-29 | E-GEOD-35436 | biostudies-arrayexpress

Profiles of IL-4 stimulated mouse peritoneal macrophages

Project description:Profiles of IL-4 stimulated mouse peritoneal macrophages

| PRJNA477285 | ENA

Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

2012-09-29 | GSE35434 | GEO

Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

2012-09-29 | GSE35433 | GEO

Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 10 ng/ml of IL-4 for 48 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

2012-09-29 | E-GEOD-35434 | biostudies-arrayexpress

Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 20 ng/ml of IL-4 for 24 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

2012-09-29 | E-GEOD-35433 | biostudies-arrayexpress

Chromatin immunoprecipitation sequencing (ChIP-Seq) for Bhlhe40 from naïve and IL-4c-stimulated large peritoneal macrophages (LPMs)

Project description:Most tissue-resident macrophage populations develop during embryogenesis, self-renew in the steady state, and can expand during type 2 immunity. Whether shared mechanisms regulate macrophage proliferation in homeostasis and disease is unclear. We found that the transcription factor Bhlhe40 was cell-intrinsically required in large peritoneal macrophages (LPMs) for self-renewal and maintenance, but was not required in other resident macrophages. Bhlhe40 was selectively necessary in LPMs for proliferation, but not polarization, in response to IL-4. During a helminth infection, Bhlhe40 was required for normal LPM cell cycling. Bhlhe40 repressed the expression of Maf and Mafb and directly promoted expression of cell cycle-related transcripts to enable proliferation of LPMs. Genomic sites bound by Bhlhe40 in LPMs included some co-bound by the macrophage lineage-determining factor PU.1 and others uniquely bound by Bhlhe40, including Maf and cell cycle-related loci. Our findings demonstrate a tissue-specific control mechanism regulating resident macrophage proliferation in homeostasis and type 2 immunity.

2019-04-30 | GSE125729 | GEO

Expression data from peritoneal macrophages stimulated with lipid A

Project description:Setdb1 is one of the H3K9 methyltransferases and represses gene expression by H3K9 methylation. In an attempt to elucidate the role of Setdb1 in the TLR4-mediated inflammatory responses, we performed DNA microarray analysis using lipid A (the active component of LPS)-stimulated peritoneal macrophages from macrophage specific Setdb1 KO (KO) and WT mice. The genes upregulated by lipid A treatment in WT macrophages and further increased in KO macrophages contain many genes associated with interleukins and chemokines. Peritoneal macrophages from WT and KO mice were stimulated with lipid A 10 ng/ml or vehicle for 4 h. Microarray analysis was performed using Affymetrix Mouse 430 2.0.

2016-06-27 | E-GEOD-78153 | biostudies-arrayexpress

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