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MicroRNA expression profiles in the esophagus of children with caustic stenosis: a pathway towards esophageal cancer?

ABSTRACT: Study Design: This study aimed to identify global microRNA (miRNA) expression changes in esophageal mucosa cells from children with caustic stenosis, divided into two groups according to the time elapsed after injury (Groups A and B, less and more than five years, respectively), compared with normal esophageal tissues from same patients. miRNA expression profiles were determined using TaqMan Human MicroRNA Arrays (Thermo Fisher). Bioinformatic algorithms were applied to identify miRNA target genes and biological pathways. Results: Thirteen miRNAs were significantly deregulated (9 over- and 4 under-expressed) in patients from Group A. In patients from Group B, two miRNAs were over- and two were under-expressed. Of note, miR-374 and miR-574 were deregulated in Group B patients and have been linked to esophageal cancer development. We identified signal transduction and transcription factor networks with genes strongly related to esophageal tumorigenesis. Conclusion:There is no literature regarding miRNA expression changes following caustic ingestion in children. miRNAs identified here may help clarify the pathway of malignant transformation from caustic stenosis to esophageal cancer and could lead to the development of novel biomarkers of malignant progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE134360 | GEO | 2020/02/27

REPOSITORIES: GEO

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Project description:Background/Objective: MicroRNAs (miRNAs) play a pivotal role in cancerogenesis and cancer progression, but their specific role in metastasis of clear cell renal cell carcinomas (ccRCCs) as so-called metastamirs is still limited. Based on microRNA microarray analyses from normal (n=12) and cancerous (n=12) samples of ccRCC specimens and from bone metastases (n=9) of ccRCC patients, we identified a set of 57 differentially expressed microRNAs between those three sample groups of ccRCC. A selected panel of 33 miRNAs, including miRNAs reported in the literature as differentially expressed in non-metastatic RCC, was validated by RT-qPCR on 57 clinical samples. 30 of the 33 examined miRNAs were confirmed to be deregulated. A stepwise down-regulated miRNA expression from normal over primary tumor to metastatic tissue samples was found to be typical. 23 miRNAs (miR-10b/-19a/-19b/-20a/-29a/-29b/-29c/-100/-101/-126/-127/-130/-141/-143/-145/-148a/-192/-194/-200c/-210/-215/-370/-514) were down-regulated in metastatic tissue samples in comparison to normal tissue. This down-regulated expression in metastatic tissue was also present in 21 miRNAs except for miR-127 and miR-370. The altered miRNA profiles including the newly identified metastasis-associated miRNAs, the compiled predicted miRNA-target interactions, and the significant correlations of miRNAs which were either lost or newly appeared in the studied sample groups afford a solid basis for further functional analyses of individual miRNAs. In this study, microarray-based profiling was performed from 9 bone metastatic tissue samples from 9 patients with clear cell renal cell carcinoma (ccRCC). For controls, 2 bone metastatic samples from two patients with prostate carcinoma and 5 total RNA pools (2 different pools of total RNAs isolated from malignant renal tissue samples derived from different ccRCC patients; 1 pool from non-malignant renal tissue of ccRCC patients; 2 pools of total RNA both from malignant and non-malignant prostate cancer tissue) were used. In addition, matched malignant (e.g., malignant NC2) and non-malignant (e.g., non-malignant NN1) samples from two independent 12 ccRCC sets were profiled (these samples were previously analyzed on a different Platform in GSE12105). The 2002 TNM System and the 2004 WHO classification was used for staging and grading.

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MicroRNA expression profiles in the esophagus of children with caustic stenosis: a pathway towards esophageal cancer?

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