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Comparison between infant and adult primary human hepatocytes (PHHs) in terms of their responsiveness to FAC (FBS + A83-01 + CHIR99021)

ABSTRACT: Infant PHHs (IPHHs) and adult PHHs (APHHs) were cultured in the presence of FBS only or FAC, and their transcriptomic changes throughout the 2-week culture were assessed by mRNA microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE134672 | GEO | 2019/08/01

REPOSITORIES: GEO

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Comparison between infant and adult primary human hepatocytes (PHHs) in terms of their responsiveness to FAC (FBS + A83-01 + CHIR99021)

Project description:Comparison between infant and adult primary human hepatocytes (PHHs) in terms of their responsiveness to FAC (FBS + A83-01 + CHIR99021)

| PRJNA556022 | ENA

Transcriptomic analysis of PHHs isolated from hCLiP-transplanted mouse chimeric liver

Project description:Transcriptome of PHHs isolated from hCLiP-transplanted mouse chimeric liver was compared with those of cryopreserved PHHs.

2019-07-04 | GSE133779 | GEO

RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)

Project description:The purpose of this experiment was to compared the transcriptome of hepatocyte-like cells (HLCs) generated in vitro and adult primary human hepatocytes (PHHs). HLCs were differentiated from either hESCs or hIPSCs using previously established protocols (Hannah et al 2013; Segeritz et al 2018). Undifferentiated hIPSCs were used as control to confirm differentiation status. PHHs were commercially sourced as well as freshly isolated from donors.

2022-08-11 | E-MTAB-10634 | biostudies-arrayexpress

Expression profile of FAC-sorted murine retinal cells

Project description:Microarray experiments were performed using FAC-sorted young photoreceptors to analyze their transcriptome in comparison to remaining retinal cells at same developmental stage and retinal progenitors. For each replicate, retinae of 6 to 8 postnatal day 0 pNestin-GFP or postnatal day 4 rhoEGFP mice were dissected and FAC-sorted based on GFP expression. RNA of fractions was isolated and subsequently analyzed with microarray experiment.

2011-07-12 | E-GEOD-29318 | biostudies-arrayexpress

Reprogramming of primary human hepatocytes (PHHs) into hCLiPs

Project description:PHHs were cultured in the presence of small molecules and converted to proliferative progenitor cells named human chemcically induced progenitors (hCLiPs).

2019-07-04 | GSE133776 | GEO

ChIP-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)

Project description:ChIP-sequencing was performed to explore the difference between the epigenetic profiles of hepatocyte-like cells (HLCs) generated in vitro and primary human hepatocytes (PHHs). HLCs were differentiated from hIPSCs and hESCs for 30 days, as previously reported (Hannan et al, 2013). PHHs were purchased from Biopredic. Chromatin was immunoprecipitated with antibodies against H3K27ac, H3K4me1, H3K27me3 and H3K4me3. Library preparation and sequencing were performed by the Wellcome Trust Sanger Institute DNA Sequencing Facility (Hinxton, UK). Sequencing was performed on an Illumina HiSeq v4 instrument to obtain paired-end reads with 75bp length.

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Francisella tularensis strain:FAC

Project description:Francisella tularensis strain:FAC Genome sequencing and assembly

| PRJNA244553 | ENA

Expression profile of FAC-sorted murine retinal cells

2011-07-12 | GSE29318 | GEO

Reprogramming of primary human hepatocytes (PHHs) into hCLiPs

Project description:Reprogramming of primary human hepatocytes (PHHs) into hCLiPs

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RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

Project description:In this report, we describe a successful protocol for isolating and expression-profiling live fluorescent- protein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. As part of this study, we also further confirm the genetic homology of mammalian and zebrafish V1 interneurons, by demonstrating that zebrafish V1 cells (CiAs) express genes that encode for the transcription factors Lhx1a and Lhx5. This protocol for dissociating, sorting and RNA-profiling neurons from organogenesis-stage zebrafish embryos should also be applicable to other developing organs and tissues and potentially other model organisms.

2020-02-26 | GSE145916 | GEO

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