Single-cell RNA-seq of mouse cells from the dorsal cerebral cortex during its formation

ABSTRACT: Dorsal cortices from embryonic day (E10.5) to postnatal day 3 (P3) were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs.

ORGANISM(S): Mus musculus

PROVIDER: GSE134738 | GEO | 2020/12/31

REPOSITORIES: GEO