Deciphering protective mechanism against human type 2 diabetes through in vitro β cell differentiation

ABSTRACT: To systemically investigate the role of ZnT8 in β cell maturation, we performed single cell RNA-seq in both WT and KO β cells at both S6 (immature) and S7 (mature) stages. Both WT and KO β cells were obtained from FACS as positive for both INS and NKX6.1. Single cell RNA-seq results revealed that SLC30A8 is mainly involved in β cell maturation process, and further showed that SLC30A8 LOF accelerates β cell maturation and upregulates insulin secretion pathway in mature β cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE135076 | GEO | 2022/05/25

REPOSITORIES: GEO