PAIR-MaP visualizes RNA base pairing complexity in human and bacterial non-coding RNAs

ABSTRACT: Structure probing experiments were performed on in vitro transcripts and E. coli and human cell cultures under natively extracted (cell-free) and in-cell conditions to benchmark the performance of the newly introduced PAIR-MaP correlated chemical probing strategy for detecting RNA duplexes. Multiple-hit dimethyl sulfate (DMS) probing was done using new buffer conditions that facilitate DMS modification of all four nucleotides.

ORGANISM(S): Escherichia coli Homo sapiens Vibrio vulnificus

PROVIDER: GSE135211 | GEO | 2019/08/01

REPOSITORIES: GEO