Transcriptomics,Genomics

Dataset Information

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RNA sequencing of CD4+ and CD8+ subsets of human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b


ABSTRACT: We report RNA sequencing data for three human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b. From total RNA from 3 biological replicates from each cell line, we generated RNA Sequencing data that compared gene expression between CD4+ and CD8+ cells for each cell line. RNA Sequencing demonstrated that the addition of full-length Helios changed gene expression in cellular pathways and the Treg signature compared to FOXP3 alone or the other major Helios isoform. Overall design: Examination of CD4+ and CD8+ cells from three human engineered regulatory T cells cell lines.

INSTRUMENT(S): Illumina NovaSeq 6000 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Mary A Markiewicz  

PROVIDER: GSE135452 | GEO | 2020-04-20

REPOSITORIES: GEO

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Publications

Coexpression of FOXP3 and a Helios isoform enhances the effectiveness of human engineered regulatory T cells.

Seng Amara A   Krausz Kelsey L KL   Pei Dong D   Koestler Devin C DC   Fischer Ryan T RT   Yankee Thomas M TM   Markiewicz Mary A MA  

Blood advances 20200401 7


Regulatory T cells (Tregs) are a subset of immune cells that suppress the immune response. Treg therapy for inflammatory diseases is being tested in the clinic, with moderate success. However, it is difficult to isolate and expand Tregs to sufficient numbers. Engineered Tregs (eTregs) can be generated in larger quantities by genetically manipulating conventional T cells to express FOXP3. These eTregs can suppress in vitro and in vivo but not as effectively as endogenous Tregs. We hypothesized th  ...[more]