Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

ABSTRACT: For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE135476 | GEO | 2019/12/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Abundances of transcripts, proteins metabolites and lipids in the cell cycle of budding yeast.

Project description:This entry contains the proteomic files from a data set of RNAs, proteins, metabolites and lipids analyzed from the same staged samples of S. cerevisiae cells across the cell cycle. Actively growing (un-arrested) diploid cells were collected by elutriation as 8 distinct size pools across the cell cycle in triplicate. Yeast in these 24 aliquots were lysed and soluble and insoluble proteins were prepared for shotgun LC-MS/MS mass spectrometry analysis.

2019-10-29 | PXD015273 | Pride

Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

Project description:Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

| PRJNA559005 | ENA

Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

Project description:cell cycle studies in the budding yeast Saccharomyces cerevisiae.

| MSV000084302 | MassIVE

Postnatal lipids drive hepatocyte maturation

Project description:Postnatal lipids drive hepatocyte maturation

| PRJNA559823 | ENA

Transcriptional impacts of lipids on hESC

Project description:Transcriptional impacts of lipids on hESC

| PRJNA698483 | ENA

Medulloblastoma cerebrospinal fluid reveals hypoxic indicators (metabolites and lipids) and cancer-specific RNAs

Project description:Medulloblastoma cerebrospinal fluid reveals hypoxic indicators (metabolites and lipids) and cancer-specific RNAs

| PRJNA785006 | ENA

Three-in-one simultaneous extraction of proteins, metabolites and lipids for multi-omics

Project description:Elucidation of complex molecular networks requires integrative analysis of molecular features and changes at different levels of information flow and regulation. Accordingly, high throughput functional genomics tools such as transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide system-wide investigations. Unfortunately, analysis of different types of biomolecules requires specific sample extraction procedures in combination with specific analytical instrumentation. The most efficient extraction protocols often only cover a restricted type of biomolecules due to their different physicochemical properties. Therefore, several sets/aliquots of samples are needed for extracting different molecules. Here we adapted a bi-phasic fractionation method to extract proteins, metabolites and lipids from the same sample (3-in-1) for liquid chromatography-tandem mass spectrometry (LC-MS/MS) multi-omics. To demonstrate utility of the improved method, we used bacteria-primed Arabidopsis leaves to generate multi-omics datasets from the same sample. In total, we were able to analyze 1849 proteins, 1967 metabolites and 424 lipid species in single samples. The molecules cover a wide range of biological and molecular processes. Our results have shown the clear advantages of the multi-omics method, including sample conservation, high reproducibility and tight correlation between different types of biomolecules.

2021-08-17 | PXD023094 | Pride

Biosynthesis of membrane-spanning lipids in Thermoanaerobacter ethanolicus

Project description:This study was conducted to identify the genes involved in the synthesis of membrane-spanning lipids in Thermoanaerobacter ethanolicus JW 200.

2022-10-26 | GSE211546 | GEO

Lipids in the development and treatment of human intestinal diseases - Lipids in intestinal diseases

Project description:Interventions: Group 1: Colon tissue (tumor, control tissue and tissue from diverticulitis patients) which is optained within a surgical intervention at the Asklepios Paulinen Clinic in Wiesbaden is directly transferred to the Clinic of Pathology of the Helios Dr.-Horst-Schmidt-Clinic Wiesbaden, where it is categorized. Within 24 h the tissue is collected from a staff of the Insitute of clinical Pharamcology University of Frankfurt. At the Institute of clinical Pharmacology of the University hospital Frankfurt the tissue is prepared for immunohistochemistry, for detection of lipids by liquid-chromatography-tandem mass spectrometry, for single cell isolation, FACS analysis, and in vitro culture experiments. Primary outcome(s): Lipid level after stimulation and anti-inflammatory therapy Study Design: Allocation: ; Masking: ; Control: ; Assignment: ; Study design purpose: basic science

| 2434471 | ecrin-mdr-crc

Transcription profiling of human enterocytes cultured with different dietary lipids

Project description:The present study aimed to identify the specific impact of dietary lipids-containing micelles on gene expression in enterocytes.

2008-08-27 | E-MEXP-1446 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data