Targeted single-cell transcriptome readouts for high-throughput genetics and functional genomics

ABSTRACT: We show that targeted single-cell RNA-sequencing (TAP-seq) permits reliable mapping of cell (sub)types with as little as 100 reads per cell and reduces the sequencing costs for differential expression testing by a factor of 10-30, thereby enabling a cost-effective profiling of a large number of genotypes at the single cell level. We demonstrate the use of TAP-seq by generating comprehensive perturbation-based enhancer-target gene maps for 1.5% of the human genome.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE135497 | GEO | 2020/03/23

REPOSITORIES: GEO