Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [mifinder Phenotypes]

ABSTRACT: Human peripheral mononuclear cells were purified from control and diseased patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

ORGANISM(S): Homo sapiens

PROVIDER: GSE135659 | GEO | 2020/10/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [miRNA Fibrosis Phenotypes]

Project description:Human peripheral mononuclear cells were purified from control and diseased (Cataract, Classical PXF, Pigmentary PXF) patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135657 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [mifinder Stages]

Project description:Human peripheral mononuclear cells were purified from control and diseased patient (Psedoexfoliation (PXF), Ocular Hypertension (OHT), Psedoexfoliation Glaucoma (PXG)) volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135660 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [miRNA Fibrosis Stages]

Project description:Human peripheral mononuclear cells were purified from control and diseased (Psedoexfoliation (PXF), Ocular Hypertension (OHT), Pseudoexfoliation Glaucoma (PXG)) patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135658 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [UPR Phenotypes]

Project description:Human peripheral mononuclear cells(PBMC) were purified from control and diseased (Cataract, Classical Pseudoexfoliation(Cl PXF), Pigmentary Pseudoexfoliation(Pig PXF)) volunteers' blood and mRNA was extracted using QIAzol® lysis reagent. complemenatry DNA(cDNA) was prepared and expression was analysed using PCR based arrays. We used QIAGEN online software to quantitate the differential gene expression .

2020-10-27 | GSE135655 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [Fibrosis Phenotypes]

2020-10-27 | GSE135653 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [UPR Stages]

Project description:Human peripheral mononuclear cells(PBMC) were purified from control and diseased volunteers' blood (Pseudoexfoliation(PXF), Ocular Hypertension(OHT), Pseudoexfoliation Glaucoma(PXG)) and mRNA was extracted using QIAzol® lysis reagent. complemenatry DNA(cDNA) was prepared and expression was analysed using PCR based arrays. We used QIAGEN online software to quantitate the differential gene expression .

2020-10-27 | GSE135656 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [Fibrosis Stages]

Project description:Human peripheral mononuclear cells(PBMC) were purified from control and diseased (Pseudoexfoliation (PXF), Ocular Hypertension(OHT), Pseudoexfoliation Glaucoma(PXG)] volunteers' blood and mRNA was extracted using QIAzol® lysis reagent. complemenatry DNA(cDNA) was prepared and expression was analysed using PCR based arrays. We used QIAGEN online software to quantitate the differential gene expression .

2020-10-27 | GSE135654 | GEO

Real-time quantitative PCR analysis of serum from osteoporotic Mexican women

Project description:RNA was purified from serum of osteoporotic and healthy postmenopausal mexican women using miRNeasy Serum/Plasma kit (QIAGEN). For microRNA expression analysis we used the Human MicroRNA A+B Cards Set v3.0 TaqMan Low Density Array platform (Applied Biosystems). Analysis was performed in the Expression Suite v.1.1.3 software (Applied Biosystems).

2018-09-11 | GSE115773 | GEO

Real-time quantitative PCR analysis of serum miRNA in response to ionizing radiation in mice

Project description:Kunming mice were total body irradiated by 0.5Gy and 2Gy carbon ions radaition. After 24 hours, blood was collected and serum was isolated. Total RNA of serum sample was extracted by miRNeasy Serum/Plasma Kit (Qiagen, Germany). Then, miRCURY LNA™ Universal RT microRNA PCR, Ready-to-Use Mouse&Rat Panel I V3 (Exiqon, Denmark) containing 384 assays was performed to profile miRNAs differential expression in these serum sample.

2016-09-10 | GSE86625 | GEO

Real-time quantitative PCR array analysis of primary colon cancer and liver metastasis derived exosomes

Project description:To isolate exosomes from colon tissue, colon tissue were removed and gently disaggregated with tweezers in dissociation buffer, followed by incubation at 37°C for 1 h. The disaggregated samples were centrifuged at 1,000 g for 10 min, 2,000 g for 20 min, 4,000 g for 30 min and 10,000 g for 1 h with the supernatant being retained each time. The tissue exosomes were collected by centrifuging the samples at 100,000 g for 1.5 h at 4°C, and the pellet was suspended in ice-cold PBS. Total RNA was isolated from cells, exosomes and tissue using a miRNeasy mini kit (Qiagen). miRNA expression profiling including exosomes and their donor cells or tissues was performed using the Qiagen miScript miRNA PCR Array Mouse miRBase Profiler. To isolate exosomes from liver tissue, a 20-G catheter was inserted into the portal vein of anaesthetized mice. The perfused liver tissue, as well as colon tissue were removed and gently disaggregated with tweezers in dissociation buffer, followed by incubation at 37°C for 1 h. The disaggregated samples were centrifuged at 1,000 g for 10 min, 2,000 g for 20 min, 4,000 g for 30 min and 10,000 g for 1 h with the supernatant being retained each time. The tissue exosomes were collected by centrifuging the samples at 100,000 g for 1.5 h at 4°C, and the pellet was suspended in ice-cold PBS. Total RNA was isolated from cells, exosomes and tissue using a miRNeasy mini kit (Qiagen). miRNA expression profiling including exosomes and their donor cells or tissues was performed using the Qiagen miScript miRNA PCR Array Mouse miRBase Profiler.

2017-01-04 | GSE93092 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data