Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast [CRAC]

ABSTRACT: CRAC analysis was performed to identify transcripts associated with the Mkt1 protein in Schizosaccharomyces pombe

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE135735 | GEO | 2019/11/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast [RNA-seq]

Project description:RNA-seq was performed to identify transcripts differentially expressed in Schizosaccharomyces pombe cells lacking Mkt1.

2019-11-28 | GSE135273 | GEO

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.

Project description: Not available

| S-EPMC7026591 | biostudies-literature

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast [CRAC]

Project description:Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast [CRAC]

| PRJNA559880 | ENA

Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe

Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains

2013-07-20 | GSE42848 | GEO

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast

Project description:Constitutive domains of repressive heterochromatin are maintained within the fission yeast genome through self-reinforcing mechanisms involving histone methylation and small RNAs. Non-coding RNAs generated from heterochromatic regions are processed into small RNAs by the RNA interference pathway, and are subject to silencing through both transcriptional and post-transcriptional mechanisms. While the pathways involved in maintenance of the repressive heterochromatin state are reasonably well understood, less is known about the requirements for its establishment. Here we describe a novel role for the post-transcriptional regulatory factor Mkt1 in establishment of heterochromatin at pericentromeres in fission yeast. Loss of Mkt1 does not affect maintenance of existing heterochromatin, but does affect its recovery following depletion, as well as de novo establishment of heterochromatin on a mini-chromosome. Pathway dissection revealed that Mkt1 is required for RNAi-mediated post-transcriptional silencing, downstream of small RNA production. Mkt1 physically associates with pericentromeric transcripts, and is additionally required for maintenance of silencing and heterochromatin at centromeres when transcriptional silencing is impaired. Our findings provide new insight into the mechanism of RNAi-mediated post-transcriptional silencing in fission yeast, and unveil an important role for post-transcriptional silencing in establishment of heterochromatin that is dispensable when full transcriptional silencing is imposed.

2019-11-28 | PXD015484 | Pride

Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast

Project description:This RNA-Seq analysis compares gene expression of the vtc4∆ mutant contrasting to the wild-type fission yeast (Schizosaccharomyces pombe).

2022-11-21 | GSE213524 | GEO

Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe

Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains Examination of a single histone modification in 4 different fission yeast strains

2013-07-20 | E-GEOD-42848 | biostudies-arrayexpress

Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

Project description:Expression profile of mutant cells compared to wild-type cells: Expression profile of Schizosaccharomyces pombe genome in ∆pht1, ∆ago1, ∆clr4, rik1, ∆pht1rik1, ∆pht1∆ago1 and ∆pht1∆clr4 cells. Expression profile of Schizosaccharomyces pombe genome in ∆rrp6, ∆cid14, ∆swi6, ∆swr1, ∆set1, ∆pht1∆swi6 and ∆pht1∆set1 cells. Expression profile of Schizosaccharomyces pombe genome in ∆alp13, ∆cph1, ∆set2, clr6-1 and ∆cph1∆pht1 cells. Occupancy profiling: Occupancy profiling of RNA polymerase II, histone variant H2A.Z and ClrC subunit Rik1 in fission yeast Schizosaccharomyces pombe Occupancy profiling of histone variant H2A.Z in ∆msc1 cells.

2009-08-25 | GSE17271 | GEO

The fission yeast Schizosaccharomyces pombe

Project description:The fission yeast Schizosaccharomyces pombe

| PRJNA413028 | ENA

Nuclear retention of fission yeast dicer is a prerequisite for RNAi-mediated heterochromatin assembly

Project description:RNAseIII ribonucleases act at the heart of RNA silencing pathways by processing precursor RNAs into mature microRNAs and siRNAs. In the fission yeast Schizosaccharomyces pombe, siRNAs are generated by the RNAseIII enzyme Dcr1 and are required for heterochromatin formation. In this study, we have analyzed the subcellular localization of Dcr1 and found that it accumulates in the nucleus and is enriched at the nuclear periphery. Nuclear accumulation of Dcr1 depends on a short motif which constrains nuclear export promoted by the double-stranded RNA binding domain of Dcr1. Absence of this motif renders Dcr1 mainly cytoplasmic and is accompanied by remarkable changes in gene expression and failure to assemble heterochromatin. Our findings suggest that dicer proteins are shuttling proteins and that the steady-state subcellular levels can be shifted towards either compartment. This has implications for the mechanism of RNAi-mediated heterochromatin assembly and the spatial organization of RNA silencing pathways in general.

2010-02-16 | GSE18582 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data