Transcriptomics,Genomics

Dataset Information

37

Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole genome tiling arrays


ABSTRACT: Plant responses to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress-induced changes in the Arabidopsis thaliana transcriptome, we have used whole-genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Overall design: After seedlings had been grown for 10 days on solid MS medium at 21°C, they were transferred to liquid MS medium containing no additives (mock control), 200 mM NaCl (salt stress), 300 mM mannitol (osmotic stress) or 100 μM ABA. For cold and heat stress, seedlings were transferred to pre-chilled or pre-warmed liquid MS medium and incubated at 8°C and 30°C, respectively. Samples were taken after 1 h and 12 h of continuous stress treatment. RNA was extracted from whole seedlings and converted into double stranded DNA targets that were hybridized to whole genome tiling arrays (Affymetrix Arabidopis Tiling1.0R®). All hybridizations were performed with 3 biological replicates.

INSTRUMENT(S): AtTile1R to Arabidopsis Tiling 1.0R

SUBMITTER: Sascha Laubinger  

PROVIDER: GSE13584 | GEO | 2008-11-14

SECONDARY ACCESSION(S): PRJNA110687

REPOSITORIES: GEO

altmetric image

Publications

Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays.

Zeller Georg G   Henz Stefan R SR   Widmer Christian K CK   Sachsenberg Timo T   Rätsch Gunnar G   Weigel Detlef D   Laubinger Sascha S  

The Plant journal : for cell and molecular biology 20090213 6


The responses of plants to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress-induced changes in the Arabidopsis thaliana transcriptome, we have used whole-genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Among annotated genes in the reference strain Columbia we have found many stress-respo  ...[more]

Similar Datasets

2010-05-08 | GSE21685 | GEO
2010-05-07 | E-GEOD-21685 | ArrayExpress
2013-05-07 | E-GEOD-46661 | ArrayExpress
2009-10-01 | GSE15063 | GEO
2009-10-01 | E-GEOD-15063 | ArrayExpress
2013-02-25 | E-GEOD-41378 | ArrayExpress
2010-01-21 | GSE19893 | GEO
2015-07-01 | E-GEOD-66946 | ArrayExpress
| GSE80567 | GEO
2008-07-01 | GSE8556 | GEO