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Transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) at harvest between contrasted years for scald incidence

ABSTRACT: Granny Smith apples (Malus x domestica Borkh) were harvested at early physiological mature stage in 2014, 2015 and 2017 and phenotyped for scald incidence after long time cold storage. Differential analyses were set-up for peel samples collected at harvest between fruit batches with low or high scald incidence.

ORGANISM(S): Malus domestica

PROVIDER: GSE135863 | GEO | 2020/04/20

REPOSITORIES: GEO

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Transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) at harvest between contrasted years for scald incidence

Project description:Transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) at harvest between contrasted years for scald incidence

| PRJNA560366 | ENA

Effect of 1-MCP and ozone post-harvest treatments on the transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage.

Project description:Granny Smith apples (Malus x domestica Borkh) grown in commercial orchards were harvested at physiological mature stage and maintained 6 months in cold storage with or without 1-MCP post-harvest treatment, and with or without enriched ozone atmosphere.

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Effect of 1-methylcyclopropene (1-MCP) and ozone (Ο 3 ) post-harvest treatments on the proteomic profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage

Project description:Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O3) induced scald symptoms in ‘Granny Smith’ apple fruits during subsequent ripening at room temperature. In contrast, treatment with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) reversed this O3-induced scald effect. Amino acids, including branched-chain amino acids, were the most strongly induced metabolites in peel tissue of 1-MCP treated fruits. Proteins involved in oxidative stress and protein trafficking were differentially accumulated prior to and during scald development. Genes involved in photosynthesis, flavonoid biosynthesis and ethylene signaling displayed significant alterations in response to 1-MCP and O3. Analysis of regulatory module networks identified putative transcription factors (TFs) that could be involved in scald. Subsequently, a transcriptional network of the genes-proteins-metabolites and the connected TFs was constructed. This approach enabled identification of several genes co-regulated by TFs, notably encoding glutathione S-transferase (GST) protein(s) with distinct signatures following 1-MCP and O3 treatments. Overall, this study is an important contribution to future functional studies and breeding programs for this fruit, aiding to the development of improved apple cultivars to superficial scald.

2020-05-27 | PXD016849 | Pride

Comparative transcriptome and metabolomic survey reveal key pathways involved in the control of the chilling injury disorder superficial scald in two apple cultivars, ‘Granny Smith’ and ‘Ladina’

Project description:The low temperature normally applied to prevent fruit decay during the storage of apples, can also triggers the onset of a chilling injury disorder known as superficial scald. In this work, the etiology of this disorder and the mechanism of action of two preventing strategies, such as the application of 1-MCP and storage at low oxygen concentration in ‘Granny Smith’ and ‘Ladina’ apple cultivars was investigated. The metabolite assessment highlighted a reorganization of specific secondary metabolites, in particular flavan-3-ols (catechin, epicatechin and procyanidin B) and unsaturated fatty acids (oleic acid, linoleic acid and linolenic acid), while the genome-wide transcriptomic analysis grouped the DEGs into four functional clusters. The KEGG pathway, GO enrichment analysis, together with the gene-metabolite profiling interactome, showed as the treatment with 1-MCP prevented the development of superficial scald by actively promoting the production of unsaturated fatty acids, especially in ‘Granny Smith’. ‘Ladina’ was instead more susceptible to superficial scald, and characterized by a higher accumulation of very long chain fatty acids (VLCFAs). Storage at low oxygen concentration stimulated a higher accumulation of ethanol and acetaldehyde together with the expression of genes involved in anaerobic respiration, such as malate and alcohol dehydrogenase and pyruvate decarboxylase in both cultivars. Moreover, in ‘Granny Smith’ apple, the expression of three members of the VII subgroups of ERF genes, encoding for elements coordinating the acclimation process to hypoxia in plants was observed. The global RNA-Seq pattern also elucidated a specific transcriptomic signature between the two cultivars, validating the effect of the different genetic background in the control of this disorder.

2023-03-29 | GSE216082 | GEO

Pre-harvest climate and post-harvest acclimation to cold prevent from superficial scald development in Granny Smith apples.

Project description: Not available

| S-EPMC7148358 | biostudies-literature

Transcriptome and metabolic survey disclose the mode of action of static and dynamic low oxygen postharvest storage strategies to prevent the onset of superficial scald disorder in fruit of ‘Granny Smith’ apple cultivar

Project description:To preserve quality feature and ensuring availability of fresh fruit on the market, apples need to be stored. The low temperature applied during storage, beside avoiding important fruit loss, can also promote the development of serious chilling injury type of disorder, such as superficial scald. One of the strategies largely employed to prevent the development of this phenomenon is the control of the storage atmosphere, by lowering down the concentration of oxygen. In this work, a multifaceted survey was carried out to investigate the variation in transcriptome together with three categories of metabolites (phenolics, lipids and volatile organic compounds, VOCs) in fruit of ‘Granny Smith’ apple cultivar stored in both static and dynamic hypoxia condition for 5 and 7 months, respectively. The global transcriptome survey identified a core set of differentially expressed genes in three main functional groups, revealing as the duration of storage had an important effect in the coordination of gene expression. The effect of the storage time was furthermore highlighted by the DEG-network analysis that identified a distinct number and type of transcriptomic hubs. Samples characterized by the development of superficial scald were distinguished by a higher concentration of chlorogenic acid and a higher expression of PAL and PPO in a time specific fashion. The prevention of this phenomenon was instead related to distinctive re-programming events, involving the accumulation of specific antioxidant types of metabolites, very long chain fatty acids and the expression of genes coordinating an hypoxia acclimation process, such as RAP2-like and PCO.

2023-12-31 | GSE225233 | GEO

Ethylene -dependent and -independent superficial scald resistance mechanisms in 'Granny Smith' apple fruit.

Project description: Not available

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Malus domestica cultivar:Granny Smith

Project description:Malus domestica cultivar:Granny Smith Raw sequence reads

| PRJNA803936 | ENA

Transcriptomic Insights on the Preventive Action of Apple (cv Granny Smith) Skin Wounding on Superficial Scald Development.

Project description: Not available

| S-EPMC8705499 | biostudies-literature

Effect of 1-MCP and ozone post-harvest treatments on the transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage.

Project description:Effect of 1-MCP and ozone post-harvest treatments on the transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage.

| PRJNA551483 | ENA

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