Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analyssis following EHMT1/2 inhibition

ABSTRACT: RNA was isolated from PEO1 olaparib resistant (PEO1-OR) cells grown in complete growth media or PEO1-OR clone 4 cells grown in complete growth media with 500 nanomolar of UNC0642 for 72 hrs. RNA-sequencing was performed to evaluate UNC0642-dependent transcriptional reprogramming, which contributes to resensitization of PEO1-OR to olaparib. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).

ORGANISM(S): Homo sapiens

PROVIDER: GSE135864 | GEO | 2019/08/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Activation of Wnt signaling promotes olaparib resistant ovarian cancer.

Project description:Sequencing of olaparib-resistant PEO1 derivatives (C4, C5, C10 and C18) and parental PEO1 (P1 and P2) cells was performed in order to determine mechanisms of acquired resistance in the resistant cell lines. PEO1 parental cell lines were authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G). Olaparib PEO1 resistant cells were generated through a step-wise escalation of olaparib (10nM to 8uM olaparib). In olaparib resistant lines an increase canonical Wnt signaling and loss of of non-canonical Wnt signaling was observed.

2018-07-28 | GSE117765 | GEO

RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

Project description:RNA-sequencing of PEO1 cells grown in suspension (SUS1 and SUS3) was performed to evaluate transcriptional reprogramming during anokis escape. CRISPR/Cas9 screen of PEO1 cells grown in adherent and suspension culture settings for 5 days or 10 days. GeCKO v2 library transduced in PEO1 cell line and cells were selected with puromycin. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).

2018-12-04 | GSE123290 | GEO

Amelioration of autism-like social deficits by targeting histone methyltransferases EHMT1/2 in Shank3-deficient mice

Project description:Many of the genes disrupted in autism are identified as histone-modifying enzymes and chromatin remodelers, most prominently those that mediate histone methylation/demethylation. However, the role of histone methylation enzymes in the pathophysiology and treatment of autism remains unknown. To address this, we used mouse models of haploinsufficiency of the Shank3 gene (a highly penetrant monogenic autism risk factor), which exhibits prominent autism-like social deficits. We found that histone methyltransferases EHMT1 and EHMT2, as well as histone lysine 9 dimethylation (specifically catalyzed by EHMT1/2), were selectively increased in the prefrontal cortex (PFC) of Shank3-deficient mice and autistic human postmortem brains. Treatment with the EHMT1/2 inhibitor UNC0642 or knockdown of EHMT1/2 in PFC induced a robust rescue of autism-like social deficits in Shank3-deficient mice, and restored NMDAR-mediated synaptic function. Activityregulated cytoskeleton-associated protein (Arc) was identified as one of the causal factors underlying the rescuing effects of UNC0642 on NMDAR function and social behaviors in Shank3-deficient mice. UNC0642 treatment also restored a large set of genes involved in neural signaling in PFC of Shank3-deficient mice. These results suggest that targeting histone methylation enzymes to adjust gene expression and ameliorate synaptic defects could be a potential therapeutic strategy for autism.

2020-07-29 | GSE155291 | GEO

Aspergillus nidulans ∆pkaA strain grown on avicel as sole carbon source

Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours

2015-07-15 | GSE70917 | GEO

Aspergillus nidulans strains TNO2A3, ∆msbA and ∆MHD grown in complete media (YUU) and on avicel as sole carbon source

Project description:Transcriptome of A. nidulans TNO2A3, ∆msbA and ∆MHD strains when grown on complete media (YUU) and transferred to minimal media plus avicel as a sole carbon source for 24 hours

2015-01-01 | GSE60594 | GEO

Aspergillus nidulans strains TNO2a3, ∆snfA and ∆schA grown on avicel as sole carbon source

Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.

2013-07-16 | GSE47810 | GEO

Whole transcriptome analysis in 661W photoreceptor cells

Project description:This experiment aimed to characterise the complete transcriptional profile of 661W cells to identify their origin as cone photoreceptor cells. Cells were either grown in complete media or subjected to 72 hours of serum starvation to encourage differentiation.

2018-08-30 | GSE119190 | GEO

Nucleosome position mapping by micrococcal nuclease analysis of S. cerevisiae cells in raffinose and galactose containing media

Project description:We analyed the nucleosome positions by using 2 concentrations of micrococcal nuclease of yeast strains that were grown in raffinose and galactose containing media (synthetic complete).

2019-05-14 | GSE116337 | GEO

Gene Expression in the Rice Blast Fungus, Magnaporthe grisea, During Nitrogen Starvation

Project description:This experiment was designed to analyze transcriptional changes that occur when the fungal plant pathogen M. grisea is shifted from a complete media to a nitrogen-starved media. Comparisons were made in the following way between fungal mycelial grown in minimal media supplemented with a nitrogen source (MM+N) and minimal media without a nitrogen source (NS) Keywords: parallel sample

2006-05-26 | GSE2716 | GEO

Cross species differential gene expression

Project description:AB SOLID sequencing of ribosome-depleted RNA from S. Cerevisiae, S. Paradoxus, S. Mikatae, and S. Bayanus These four yeast species were grown in complete media and total RNA was sequenced.

2011-12-31 | GSE32679 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data