Project description:The goal of this study is to uncover the changes in the transcriptome of sensory neurons of the liver kinase B1 (LKB1) knockout

2020-03-11 | GSE146756 | GEO

Molecular characterisation of sorted populations of DRG and TG ganglia neurons

Project description:Sensory neurons were extracted from the dorsal root or trigeminal ganglia of AdvGFP transgenic mice and their molecular profile analysed via RNA-seq.

2017-10-11 | GSE100175 | GEO

GPCR expression in mouse dorsal root ganglia (DRG)

Project description:RNA of dorsal root ganglia from untreated adult mice (6-8 weeks) was tested for GPCR expression

2015-12-01 | GSE75476 | GEO

Profiling of visceral sensory neurons in dorsal root and nodose ganglia in BALB/c mice.

Project description:Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation. Keywords: Cell type comparison

2005-12-21 | GSE2917 | GEO

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