Mapping PT modifications in bacteria

ABSTRACT: One microbial organism always contains multiple DNA modification systems as defense strategies or epigenetic controls. Here, we describe crosstalk between two distinct DNA modifications with different target sequence motif: DNA phosphorothioation (PT, GPSAAC/GPSTTC by DndACDE proteins) and N6-methyl-adenine methylation (m6A, G6mATC by Dam protein). By using a newly developed DNA modification sequencing method PT nick-seq, we found that half of PT modifications change from GAAC/GTTC to GATC sites after bacteria have lost Dam.

ORGANISM(S): Salmonella enterica Escherichia coli K-12

PROVIDER: GSE135910 | GEO | 2020/05/21

REPOSITORIES: GEO