Transcriptomics

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T7-based linear amplification of low concentration mRNA samples using beads and microfluidics


ABSTRACT: We have demonstrated in vitro transcription (IVT) of cDNA sequences from purified Jurkat T-cell mRNA immobilization on microfluidic packed beads down to single-cell quantities. The microfluidic amplified aRNA was nearly identical in length and quantity compared with benchtop reactions using the same starting sample quantities. Microarrays were used to characterize the number and population of genes in each sample, allowing comparison of the microfluidic and benchtop processes. Keywords: gene expression transcripts detected

ORGANISM(S): Homo sapiens

PROVIDER: GSE13595 | GEO | 2009/01/01

SECONDARY ACCESSION(S): PRJNA110707

REPOSITORIES: GEO

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