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NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque

ABSTRACT: In this study, we aim to establish the means to characterize macaque natural killer (NK) cells, and to investigate whether the CD27/CD11b NK maturation model proposed in human and murine models is applicable to Macaca fascicularis (cynomolgus macaque) NK cells. The NK development studies performed in mice models have demonstrated four functionally distinct subsets defined by CD27 and CD11b, however this has not yet been established in nonhuman primates. The expression data of these macaque NK subsets will be associated with the antibody-dependent cellular cytotoxicity and missing-self responses produce by each subset.

ORGANISM(S): Macaca fascicularis Macaca mulatta

PROVIDER: GSE136051 | GEO | 2019/12/06

REPOSITORIES: GEO

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NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque.

Project description:In this study, we aim to establish the means to characterize cynomolgus macaque natural killer (NK) cells, and to investigate the CD27/CD11b NK maturation model in Macaca fascicularis NK cell subpopulations. The NK development studies performed mainly in mice models have demonstrated four functionally distinct subsets as defined by CD27 and CD11b, however this has not yet been established in nonhuman primates. The expression data of these macaque NK subsets will be associated with the antibody-dependent cellular cytotoxicity and missing-self responses produce by each subset.

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Project description:Previous reports have defined three subsets of mouse NK cells on the basis of the expression of CD27 and CD11b. The developmental relationship between these subsets was unclear. To address this issue, we evaluated the overall proximity between mouse NK cell subsets defined by CD27 and CD11b expression using pangenomic gene expression profiling. The results suggest that CD27+CD11b-, CD27+CD11b+ and CD27-CD11b+ correspond to three different intermediates stages of NK cell development. Experiment Overall Design: Spleen cells from RAG-/- mice have been isolated and stained with anti-NK1.1, anti CD27 and anti CD11b antibodies. NK1.1+ cells were sorted into CD27+ CD11b-, CD27+ CD11b+ and CD27- CD11b+ subsets by flow cytometry. There are two independent replicates for each sample. Total RNA was extracted with the RNeasy microkit (Qiagen) and gene expression profiles were performed according to manufacturer instructions (Affymetrix mouse 430 2.0).

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NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque.

Project description:NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque.

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NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque

Project description:NK cells expressing CD27 and CD11b are mature NK intermediates with organ specific distribution in cynomolgus macaque

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Project description:The cell lineage origin of interferon-producing killer dendritic cells (IKDC), which exhibit prominent anti-tumoral activity, has been subject to debate. Although IKDC were first described as a cell type exhibiting both pDC and NK cell properties, the current view reflects that IKDC merely represent activated NK cells expressing B220, which were thus renamed B220+ NK cells. Herein, we further investigate the lineage relationship of B220+ NK cells with regards to other NK cell subsets. We surprisingly find that, upon adoptive transfer, B220- NK cells did not acquire B220 expression, even in the presence of potent activating stimuli. These findings strongly argue against the concept that B220+ NK cells are activated NK cells in vivo. Moreover, we unequivocally demonstrate that B220+ NK cells are highly proliferative and differentiate into mature NK cells upon in vivo adoptive transfer. Additional phenotypic, functional and transcriptional characterizations further define B220+ NK cells as immediate precursors to mature NK cells. By analogy to pre-B cells and pre-DC, we propose that B220+ NK cells should be renamed pre-NK cells. The characterization of these novel attributes to pre-NK cells will guide the identification of their ortholog in humans, contributing to the design of potent cancer immunotherapies. Total RNA from B220+ NK cells compared to total RNA from CD27- and CD27+ NK cell subsets all isolated ex vivo from the spleen of B6.Rag-/-. Triplicatas were analysed.

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2016-04-01 | E-GEOD-45842 | biostudies-arrayexpress

Expression profiles of mouse NK cell subsets

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Id2-deficient NK cells acquire a naïve-like fate (Affymetrix data set)

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