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Bulk RNA-Seq for MDSCs

ABSTRACT: Myeloid-derived suppressor cells (MDSCs) as a population of myeloid cells enriched in cancer patients with immunosuppressive function. Further studies have determined that MDSCs are comprised of two groups: polymorphonuclear-MDSCs (G-MDSCs) and monocytic-MDSCs (M-MDSCs). We used a microbeads kit (Miltenyi) to isolate G-MDSCs and M-MDSCs with markers Ly6c2-Ly6g+CD11b+ and Ly6c2+Ly6g-CD11b+, respectively. And then using bulk RNA-seq to anlysis the enrichment gene expression in G-or M-MDSC which from wild type, APCmin mutant adenoma and immune deffiency mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE136258 | GEO | 2022/08/21

REPOSITORIES: GEO

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Effects of CXCR2 knockdown in gastric granulocytic myeloid-derived suppressor cells (G-MDSCs) during chronic inflamation.

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