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Gene expression analysis of the wheat response to infection by Fusarium pseudograminearum

ABSTRACT: Crown rot of wheat, caused by Fusarium pseudograminearum and other Fusarium species is an important disease globally. To understand the host response to challenge by Fp, we examined gene exression changes in the stem base of the wheat variety Kennedy, following inoculation with macroconidia using the Affymetrix GeneChip Wheat Genome Array. Induced genes included mainly those with defensive functions such as genes encoding anti-microbial proteins as well as oxidative stress-related proteins, signalling molecules, and proteins involved in both primary and secondary metabolism. This study is the first comprehensive analysis of the wheat transcriptome during crown rot infection and provides new insights into the host processes involved in plant defence against this pathogen.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE13660 | GEO | 2009/01/01

SECONDARY ACCESSION(S): PRJNA110211

REPOSITORIES: GEO

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Transcription profiling of wheat response to infection by Fusarium pseudograminearum

Project description:Crown rot of wheat, caused by Fusarium pseudograminearum and other Fusarium species is an important disease globally. To understand the host response to challenge by Fp, we examined gene exression changes in the stem base of the wheat variety Kennedy, following inoculation with macroconidia using the Affymetrix GeneChip Wheat Genome Array. Induced genes included mainly those with defensive functions such as genes encoding anti-microbial proteins as well as oxidative stress-related proteins, signalling molecules, and proteins involved in both primary and secondary metabolism. This study is the first comprehensive analysis of the wheat transcriptome during crown rot infection and provides new insights into the host processes involved in plant defence against this pathogen. Experiment Overall Design: There are six samples, three F. pseudograminearum inoculated samples and three mock inoculated samples. Each sample consists of 2cm of stem base from approximately 20 plants.

2009-01-14 | E-GEOD-13660 | biostudies-arrayexpress

Triticum aestivum Transcriptome or Gene expression

Project description:Identify genes underlying the Fusarium crown rot resistance locus and find out if expressed genes associated with resistance to Fusarium crown rot were related to those observed by others for Fusarium head blight.

2015-10-15 | E-MTAB-4308 | biostudies-arrayexpress

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TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

Project description:Abstract Background: Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. Results: Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. Conclusion: The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.

2021-01-04 | PXD023314 | Pride

Maize gene expression during infection with Fusarium moniliforme

Project description:The fungal pathogen Fusarium moniliforme causes ear rot in maize. Ear rot in maize is a destructive disease globally caused by Fusarium moniliforme , due to decrease of grain yield and increase of risks in raising livestock by mycotoxins production. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Fusarium moniliforme in its host plant to get insights into the defense programs and the host processes potentially involved in plant defense against this pathogen.

2009-12-16 | GSE19501 | GEO

Wheat pathogen Fusarium pseudograminearum CS3487 genome sequencing

Project description:Fusarium pseudograminearum CS3487 pathogen recovered from crown-rot infected wheat fields

| PRJEB1734 | ENA

Wheat pathogen Fusarium pseudograminearum CS3220 genome sequencing

Project description:Fusarium pseudograminearum CS3220 pathogen recovered from crown-rot infected wheat fields

| PRJEB1727 | ENA

Wheat pathogen Fusarium pseudograminearum CS3427 genome sequencing

Project description:Fusarium pseudograminearum CS3427 pathogen recovered from crown-rot infected wheat fields

| PRJEB1733 | ENA

Wheat pathogen Fusarium acuminatum CS5907 genome sequencing

Project description:Fusarium acuminatum CS5907 pathogen recovered from crown-rot infected wheat fields

| PRJEB1737 | ENA

Wheat pathogen Fusarium pseudograminearum CS5834 genome sequencing

Project description:Fusarium pseudograminearum CS5834 pathogen recovered from crown-rot infected wheat fields

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