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Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE136811 | GEO | 2020/04/07

REPOSITORIES: GEO

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Nuclear interacting SET domain protein 1 inactivation impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

Project description: Not available

| S-EPMC7293310 | biostudies-literature

Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia

Project description:Nuclear receptor binding SET domain protein 1 (NSD1) is recurrently mutated in human cancers including acute leukemia. We found that NSD1 knockdown altered erythroid clonogenic growth of human CD34+ hematopoietic cells. Ablation of Nsd1 in the hematopoietic system induced a transplantable erythroleukemia in mice. Despite abundant expression of the transcriptional master regulator GATA1, in vitro differentiation of Nsd1-/- erythroblasts was majorly impaired associated with reduced activation of GATA1-induced targets, while GATA1-repressed target genes were less affected. Retroviral expression of wildtype Nsd1, but not a catalytically-inactive Nsd1N1918Q SET-domain mutant induced terminal maturation of Nsd1-/- erythroblasts. Despite similar GATA1 levels, exogenous Nsd1 but not Nsd1N1918Q significantly increased GATA1 chromatin occupancy and target gene activation. Notably, Nsd1 expression reduced the association of GATA1 with the co-repressor SKI, and knockdown of SKI induced differentiation of Nsd1-/- erythroblasts. Collectively, we identified the NSD1 methyltransferase as a novel regulator of GATA1-controlled erythroid differentiation and leukemogenesis.

2020-04-08 | PXD017657 | Pride

Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia. NSD1 Add-back experiment, mRNASeq cultured erythroblasts.

Project description:In vitro differentiation of Nsd1-/- erythroblasts with retroviral expression of wildtype Nsd1, or a catalytically-inactive Nsd1N1918Q SET-domain

2020-04-07 | GSE136810 | GEO

Project description:In vitro differentiation of Nsd1-/- erythroblasts with retroviral expression of wildtype Nsd1, or a catalytically-inactive Nsd1N1918Q SET-domain

2020-04-07 | GSE136808 | GEO

Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia. KO experiement, mRNASeq whole bone-marrow.

Project description:Bone marrow of lin-neg erythroblasts either diseased Nsd1-/- or healthy Nsd1fl/fl

2020-04-07 | GSE136809 | GEO

Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

Project description:Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

| PRJNA563705 | ENA

Project description:Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia. NSD1 Add-back experiment, mRNASeq cultured erythroblasts.

| PRJNA563709 | ENA

| PRJNA563707 | ENA

Inactivation of the nuclear interacting SET domain protein 1 (NSD1) impairs GATA1-regulated erythroid differentiation and causes erythroleukemia. KO experiement, mRNASeq whole bone-marrow.

| PRJNA563708 | ENA

FAM122A Inhibits Erythroid Differentiation through GATA1.

Project description: Not available

| S-EPMC7486200 | biostudies-literature