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NRF2 is a key regulator of endothelial miRNA expression under pro-atherogenic stimuli

ABSTRACT: Regulation of coding and non-coding genes is studied from primary human aortic endothelial cells (HAECs), venous endothelial cells (HUVECs), aortic smooth muscle cells (HASMCs) and macrophages (CD14+) under pro-atherogenic stimuli (hypoxia, oxPAPC and hypoxia+oxPAPC) by integrating three different sequencing techinques: GRO-seq, miRNA-seq and RNA-seq

ORGANISM(S): Homo sapiens

PROVIDER: GSE136813 | GEO | 2020/06/17

REPOSITORIES: GEO

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Proatherogenic gene regulation explained by cell-type-specific miRNA profiling

Project description:Regulation of coding and non-coding genes is studied from primary human aortic endothelial cells (HAECs), venous endothelial cells (HUVECs), aortic smooth muscle cells (HASMCs) and macrophages (CD14+) under pro-atherogenic stimuli (hypoxia, oxPAPC and hypoxia+oxPAPC) by integrating three different sequencing techniques: GRO-seq, miRNA-seq and RNA-seq.

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Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function

Project description:Oxidation products of 1-palmitoyl-2-arachidonoyl-sn-glycerol-2-phosphatidylcholine (PAPC), referred to as OxPAPC, accumulate in atherosclerotic lesions and regulate over 1000 genes in human aortic endothelial cells (HAEC). We previously demonstrated that OxPAPC covalently binds to a number of proteins in HAEC. The goal of these studies was to gain insight into the binding mechanism and determine if binding regulates activity. In whole cells N-acetylcysteine inhibited gene regulation by OxPAPC, and blocking cell cysteines with N-ethylmaleimide strongly inhibited OxPAPC binding. Using MS, we demonstrate that most of the binding of OxPAPC to cysteine is mediated by PEIPC. We also show that OxPAPC binds to a model protein, H-Ras, at cysteines previously shown to regulate activity in response to 15dPGJ2. This binding was observed with recombinant protein and in cells overexpressing H-Ras. 15dPGJ2 and OxPAPC increased H-Ras activity at comparable concentrations. Using microarray analysis, we demonstrate a considerable overlap of gene regulation by OxPAPC and 15dPGJ2 in HAEC, suggesting that some effects attributed to 15dPGJ2 may also be regulated by OxPAPC since OxPAPC lipids and 15dPGJ2 both accumulate in inflammatory sites. Overall, we provide evidence for the importance of OxPAPC-cysteine interactions in regulating HAEC function. Control, OxPAPC, and 15dPGJ2-treated HAECs Samples. 'Control' is media (1%FBS, 99%M199)-only treated HAECs. 15dPGJ2 and OxPAPC are treated with those respective compounds in media.

2012-02-14 | E-GEOD-35709 | biostudies-arrayexpress

ChIP-seq of H3K27acethylation of human aortic endothelial cells (HAECs) treated with Cobalt chloride (CoCl2)

Project description:Endothelial-to-mesenchymal transition (EndMT) is a fundamental process in vascular remodeling, and hypoxia is known to induce EndMT and involved in cardiovascular disease development. Cobalt chloride (CoCl2), a chemical inducer of hypoxia-inducible factor-1 (HIF-1) could influence cellular function and its differentiation. However, exact molecular mechanism how stabilization of HIF-1 by cobalt chloride in human primary ECs affects cellular behavior and EndMT process is largely unknown. In this study, we performed ChIP-seq for H3K27ac in human aortic endothelial cells (HAECs) treated with CoCl2

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Effect of H2O2 and Collagen tripeptide (CTP) on endothelial cells and smooth muscle cells in cell culture

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The role of TERF2IP (Telomeric repeat binding factor 2-interacting protein) in regulating disturbed flow-induced changes of gene expression

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Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function

2012-02-14 | GSE35709 | GEO

NRF2 is a key regulator of endothelial miRNA expression under pro-atherogenic stimuli

Project description:NRF2 is a key regulator of endothelial miRNA expression under pro-atherogenic stimuli

| PRJNA563721 | ENA

Allele-specific 4C-seq of rs2836411 located in the ERG gene in HUVEC and HASMCs

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2019-10-11 | GSE137288 | GEO

RNA-Seq of human aortic endothelial cells with lysophosphatidylinositol against untreated controls

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