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Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase

ABSTRACT: 7,8-dihydro-8-oxoguanine (OG) is one of the major DNA oxidative damages, which has been concerned by researchers for its potential of mutagenesis and epigenetic regulation. Genome-wide mapping of oxidative DNA damage is of great value for understanding the biological impact of these modifications. Here we present a novel appoach to profile guanine oxidation in the mouse genome. Through structure-guided mutagenesis, we've successufully engineered 8-oxoguanine DNA glycosylase into a useful tool for OG detection. By enrichment of glycosylase-DNA complex, thousands of OG peaks were identified in the mouse genome.

ORGANISM(S): Mus musculus

PROVIDER: GSE136862 | GEO | 2019/10/18

REPOSITORIES: GEO

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Expression data from human fibroblasts 6 and 12 hours after 8-oxoguanine treatment

2011-01-27 | GSE26813 | GEO

Expression data from human fibroblasts 6 and 12 hours after 8-oxoguanine treatment

Project description:8-oxoguanine is excised from DNA by 8-oxoguanine DNA glycosylase-1 during DNA base excision repair. We used microarrays to gain insight into the potential biological function of the excised, free 8-oxoguanine base. MRC5, human diploid fibroblasts were exposed to 8-oxoguanine and changes in gene expression compared to mock control were analyzed 6 hours after exposure. MRC5, human diploid fibroblasts were exposed to 8-oxoguanine and changes in gene expression compared to mock control were analyzed 12 hours after exposure.

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Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase

Project description:Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase

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2014-01-13 | E-GEOD-54007 | biostudies-arrayexpress

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Project description:Genome-wide analysis of 8-oxoguanine DNA glycosylase-1 after oxidative stress exposure

| PRJNA349805 | ENA

Real-time quantitative PCR analysis of mouse lungs

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