Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase

ABSTRACT: 7,8-dihydro-8-oxoguanine (OG) is one of the major DNA oxidative damages, which has been concerned by researchers for its potential of mutagenesis and epigenetic regulation. Genome-wide mapping of oxidative DNA damage is of great value for understanding the biological impact of these modifications. Here we present a novel appoach to profile guanine oxidation in the mouse genome. Through structure-guided mutagenesis, we've successufully engineered 8-oxoguanine DNA glycosylase into a useful tool for OG detection. By enrichment of glycosylase-DNA complex, thousands of OG peaks were identified in the mouse genome.

ORGANISM(S): Mus musculus

PROVIDER: GSE136862 | GEO | 2019/10/18

REPOSITORIES: GEO