Individual nucleotide resolution Crosslinking Immunoprecipitation (iCLIP)-seq

ABSTRACT: Purpose: to identify the AGO1-bound miRNAs and mRNAs in human endothelial cells subject to hypoxic condition (2% oxygen) Methods: human microvascular endothelial cells was cultured under normoxia (20% oxygen) or hypoxia (2%) oxygen for 24 hours. Total RNA was extracted and RNA and small RNA libraries were prepared for Seq. Results: We found substantial changes in AGO1-bound mRNAs due to hypoxia. A portion of CLIP-seq reads also reveal direct binding to miRNA to mRNA. Conclusions: hypoxia induces profound changes in mRNAs associated with AGO1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE136907 | GEO | 2019/09/06

REPOSITORIES: GEO