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RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

ABSTRACT: Purpose: to profile transcriptome changes due to EC-AGO1-deficiency Methods: Subcutaneous adipose tissue were harvested. RNA was extracted using TRIzol and library was prepared for sequencing. Results: We found genes involved in pathways promoting angiogenesis, browning, insulin sensitivity to be upregulated, and those promoting inflammation and fibrosis to be decreased in KO compared with WT mice. Conclusions:Our study represents the detailed analysis of Ago1 regulate transcriptomes in SAT between WT and KO mice. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

ORGANISM(S): Mus musculus

PROVIDER: GSE136910 | GEO | 2019/09/06

REPOSITORIES: GEO

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small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

Project description:Purpose:to reveal the changes in small RNA, esp. microRNA to correlate with transcriptome changes occuring due to EC-AGO1-KO and further explain the observed phenotype. Methods: Subcutaneous adipose tissue were harvested.Small RNA library was prepared and for sequencing. Results: We found substantial changes in miRNA due to EC-AGO1-KO. Conclusions: Our study represents the detailed analysis of Ago1 regulated small RNA transcriptomes in subcutaneous adipose tissue between WT and KO mice. We conclude that small RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

2019-09-06 | GSE136911 | GEO

Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

Project description:Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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RNA Seq of HMVEC under hypoxia

Project description:Purpose: to profile transcriptome changes due to EC-AGO1-deficiency Methods: We had HMVECs subjected to normoxia (20%) or hypoxia (2%) for 0, 12, 24, and 48 hr in biological replicates. Total RNA was extracted with mirVana total RNA isolation kit (Life Technologies). RNA-seq was performed. Results: We found genes involved in pathways promoting angiogenesis, browning, insulin sensitivity to be upregulated, and those promoting inflammation and fibrosis to be decreased in KO compared with WT mice. Conclusions:Our study represents the detailed analysis of Ago1 regulate transcriptomes in HMVEC under Hx. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. To elucidate the molecular changes underlying the observed metabolic phenotype in EC-AGO1-KO mice.

2019-09-06 | GSE136908 | GEO

RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

Project description:RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-09-06 | GSE136912 | GEO

small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

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Gene expression profiling in cells with Ago1 knockdown (Affymetrix)

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2013-09-27 | E-GEOD-42600 | biostudies-arrayexpress

Gene expression profiling in cells with Ago1 knockdown (Affymetrix)

2013-09-27 | GSE42600 | GEO

Small RNA profiling of WT E14, Ago2_KO and Ago2_KO-AGO1 mouse embryonic stem cells.

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Consequences of depleting core RNAi factors on miRNA profiles

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