Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Donor-cell memory confers metastable state of directly converted cells

ABSTRACT: How the phenotypic instability of directly converted cells influences in vivo functional consequences is largely unknown. Here we show that the directly converted iOPCs exhibit a metastable state, such that they can progress to mature oligodendrocytes or revert back to the donor-cell type in a context-dependent fashion. Thus, therapeutic use of such cells will require a more detailed understanding of mechanisms by which the fate of directly converted cells may be rendered durable.

ORGANISM(S): Mus musculus

PROVIDER: GSE137907 | GEO | 2022/09/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression analysis of directly converted brown adipocytes (dBAs). [human]

Project description:Comparasion of each cell mRNA expression pattern Human fibroblasts were directly converted into brown adipocytes (dBAs) by transducing some transcription factors. To characterize the dBAs more in detail, RNA extracted from the human WAs, human dBAs, and human iPS-derived brown adipocytes (iBAs) were subjected to DNA microarray analysis, and global gene expression profiles of the cells were compared.

2015-08-12 | GSE56633 | GEO

Gene expression analysis of directly converted brown adipocytes (dBAs). [mouse]

Project description:Comparasion of each cell mRNA expression pattern Mouse fibroblasts were directly converted into brown adipocytes (dBAs) by transducing some transcription factors. To characterize the dBAs more in detail, RNA extracted from the mouse brown adipose tissue, mouse dBAs, and mouse iPS-derived brown adipocytes (iBAs) were subjected to DNA microarray analysis, and global gene expression profiles of the cells were compared.

2015-08-12 | GSE56634 | GEO

Gene expression analysis of directly converted brown adipocytes (dBAs). [mouse]

2015-08-12 | E-GEOD-56634 | biostudies-arrayexpress

Gene expression analysis of directly converted brown adipocytes (dBAs). [human]

2015-08-12 | E-GEOD-56633 | biostudies-arrayexpress

Methylation profiling of directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL) to study age-associated changes.

Project description:This study comprises DNA methylation profiles of 9 directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL).

2024-01-01 | GSE247703 | GEO

Genetic and epigenetic variation among inbred littermates: identification of metastable epialleles in the mouse

Project description:Genomic DNA was extracted from the livers of 5 22week old male mice, bisulfite converted and sequenced to ~30x coverage. The mice were obtained from two litters from an inbred agouti viable yellow colony. The individuals were isogenic but differed in their coat phenotype with one having a yellow coat, one a psuedoagouti coat and the remaining were phenotypically C57/BL6. The resulting DNA metylation profiles were investigated for individual differences in DNA methylation, including the identificaiton of novel metastable epialleles.

2015-12-01 | GSE72177 | GEO

Genome-wide expression analysis of human fibroblasts and converted neurons

2014-01-16 | GSE45954 | GEO

An expression microarray approach for the identification of candidate metastable epialleles in the mouse genome

Project description:Genetic loci displaying environmentally responsive epigenetic marks, termed metastable epialleles, offer a solution to the paradox presented by genetically identical yet phenotypically distinct individuals. The murine viable yellow agouti (Avy) locus is a well-described metastable epiallele that serves as a visual epigenetic biosensor. The Avy locus exhibits a high R-value or ratio of inter-individual (Vi) to inter-tissue (Vt) variance in gene expression, characteristic of what we term the ‘Agouti Expression Fingerprint.’ We propose a novel method for identification of candidate metastable epialleles based on the Agouti Expression Fingerprint, defining candidates as loci with R-values greater than 1.5 on expression microarray. Using Expression data from tissues of the three germ layers (liver, kidney, brain), high variance in agouti RNA levels among isogenic animals coupled with low variance among tissue types in individual animals is demonstrated. Here, we provide proof of concept for the ‘Agouti Expression Fingerprint’; the characterization of epigenetically labile loci in humans will be crucial to the development of novel screening and therapeutic targets for human disease prevention. For expression microarray studies, total RNA was isolated from liver, kidney, and brain tissue from 10 male Avy/a mice (2 per each of the 5 coat color classes) at time of weaning and coat color determination (day 22). Using Affymetrix GeneChip Mouse Genome 2.0 arrays (Santa Clara, CA), we queried the entire mouse genome for candidate metastable epialleles that display the Agouti Fingerprint. Approximately 100 of the greater than 40,000 transcripts on the mouse array displayed an expression pattern characterized as high inter-individual variation coupled with low inter-tissue variation (R-value > 1.5).

2011-07-14 | E-GEOD-28559 | biostudies-arrayexpress

Genome-wide expression analysis of human fibroblasts and converted neurons

Project description:Human fibroblasts can be directly converted into cholinergic neurons by Neurogenin 2 (Neurog2 or NGN2) under the treatments of small molecules. Genome-wide analysis of gene expression was performed to examine the similarity of converted neurons to samples from human brain or spinal cord. Total RNA obtained from isolated human fetal lung fibroblasts or converted neurons at 21 days. Commercially available total RNAs from adult human brains and spinal cords were used as controls.

2014-01-16 | E-GEOD-45954 | biostudies-arrayexpress

An expression microarray approach for the identification of candidate metastable epialleles in the mouse genome

2011-07-15 | GSE28559 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data