Dataset Information

Transcriptome profiles in the catkins of two Castanea mollissima cultivars for dissecting the mechanisms of Shorter Catkins Mutation.

ABSTRACT: Chinese Chestnut (Castanea mollissima BL.) has high nutritional and ecological value, so it is widely planted.However, the number of male flowers far exceeds the number of female flowers is an important factor limiting the yield of Chinese chestnut.A naturally occurring mutation in male catkin has been found on a Chinese chestnut tree in the mountains of Beijing, China.This mutant is shorter than the normal catkin.Studies have shown that this mutation can significantly reduce the number of male flowers, promote the differentiation of female flowers, and improve the yield of Chinese chestnut.In this research, we provided the RNA sequencing data of two groups of mutants and normal catkin.The comparative analyses of catkins transcriptomes presented here provides a valuable resource for discovery of genes and networks involved in the regulate the sex of the flower.

ORGANISM(S): Castanea mollissima

PROVIDER: GSE137937 | GEO | 2019/09/25

REPOSITORIES: GEO

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Project description:Cucurbita maxima c.v. Big Max is a monoecious plant with separate male and female flowers which utilizes nectar as a floral reward for the attraction of bees to the flowers. Maintenance of this mutualism with bees is essential for the reproductive success in this economically valuable crop. In this study, the sex-dependent variation in composition of male and female nectar and the nectaries were defined using a combination of GC-MS based metabolomics and LC-MS/MS based proteomics. Nectar proteins were detected non-quantitatively using LC-MS/MS coupled to Orbitrap. The male and female nectary proteomes were quantified using iTRAQ labeled peptides which were fractioned and concatenated prior to detection using LC-MS/MS coupled to Orbitrap. The nectar proteome consists of 46 proteins, of which 70% overlap between nectar types. Only two proteins are unique to female nectar, and 10 are specific to male nectar. Nectarins (nectar proteins) commonly alter nectar carbohydrates. In our datasets, significant differences in carbohydrate abundance, specifically galactose and sucrose, may be explained by the unique presence of galactinol--sucrose galactosyltransferase 2 (functioning in galactose metabolism) and invertase (catalyzes the hydrolysis of sucrose to glucose and fructose) in the nectar of female and male flowers respectively. The nectary proteome contained 339 identifiable proteins, 71% of which were descriptively annotatable by homology to Plantae. The abundance of 45 proteins differs significantly between male and female nectaries. GO slim analysis revealed a high abundance of proteins related to transport, protein metabolism, carbohydrate metabolism, response to stress, and amino acid metabolism. Comparisons of relative protein abundance revealed that 45 proteins displayed differential expression between male and female nectaries (p-value <0.05); 20 of these proteins were more abundant in male nectaries and 25 were more abundant in female nectaries. GO enrichment of nectary proteins with increased female abundances indicate that female-enriched GO terms are associated with proteins functioning as plasma membrane proton pumps and central metabolism. Proteins associated with male nectary-enriched GO terms were related to cinnamic acid biosynthesis and neutralization of superoxide radicals and hydrogen peroxide.

Dataset Information

Transcriptome profiles in the catkins of two Castanea mollissima cultivars for dissecting the mechanisms of Shorter Catkins Mutation.

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