Linker-mediated PCR for global integration site analysis in splenic human CD4-positive T cells of HIV1-GFP-infected humanized mice

ABSTRACT: To comprehensively compare the HIV-1 integration sites between viral producing cells (GFP+ cells) and non-producing cells (GFP- cells), linker-mediated PCR is performed by using the samples obtained from HIV1-GFP-infected humanized mice. Additionally, to reactivate latently infected cells, viral non-producing cells (GFP- cells) were stimulated with PMA and ionomycin ex vivo. Then, the viral integration sites in potential latently infected cells (GFP-_GFP+ cells) and the cells with dead/inactivated proviruses (GFP-_GFP- cells) were analyzed by linker-mediated PCR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE137962 | GEO | 2020/07/16

REPOSITORIES: GEO