Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Interaction between the light environment and the Arabidopsis wound response

ABSTRACT: We have previously identified a significant increase in chloroplast reactive oxygen species in wounded leaves of Arabidopsis and other plants, which is light-dependent (Flor-Henry et al. (2004) BMC Plant Biology 4:19). The aims of this study were to (i) examine the early response to mechanical wounding in Arabidopsis leaves, (ii) test the hypothesis that light-dependent chloroplast ROS may play a role in signalling for changes in gene expression in wounded leaves, and (iii) examine the broader impact of the light environment on the wound response in Arabidopsis. Keywords: Stress response

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE13803 | GEO | 2010/04/22

SECONDARY ACCESSION(S): PRJNA110567

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Interaction between the light environment and the Arabidopsis wound response

Project description:We have previously identified a significant increase in chloroplast reactive oxygen species in wounded leaves of Arabidopsis and other plants, which is light-dependent (Flor-Henry et al. (2004) BMC Plant Biology 4:19). The aims of this study were to (i) examine the early response to mechanical wounding in Arabidopsis leaves, (ii) test the hypothesis that light-dependent chloroplast ROS may play a role in signalling for changes in gene expression in wounded leaves, and (iii) examine the broader impact of the light environment on the wound response in Arabidopsis. Keywords: Stress response The experiment was a two-by-two factorial design, with wounding and light as the two variables. In each replicate, there are 4 treatments: unwounded/light, unwounded/dark, wounded/light, wounded/dark. Three independent biological replicate experiments were performed, with one array hybridisation performed per treatment.

2010-05-07 | E-GEOD-13803 | biostudies-arrayexpress

BrphyB is critical for rapid recovery to darkness in mature Brassica rapa leaves

Project description:Here, we examine the transcriptomic response of adult wild-type and BrphyB leaves to darkening and recovery in light. Three days of dark was sufficient to elicit a response in wild type leaves suggesting a shift from carbon fixation and nutrient acquisition to active redistribution of cellular resources. Upon a return to light, wild-type leaves appeared to transcriptionally return to a pre-darkness state restoring a focus on nutrient acquisition. BrphyB mutant plants have a similar response with key differences in genes involved in photosynthesis and light response which deviate from the wild type transcriptional dynamics. Genes targeted to the chloroplast are especially affected. Adult plants had fewer, larger chloroplasts suggesting a link between phytochromes, chloroplast development, photosynthetic deficiencies and resource allocation.

2020-08-18 | GSE135955 | GEO

wt and opr3 control and systemic wounding

Project description:wt plants (ws) and opr3 mutant plants were wounded Half of the rosette leaves of 6 weeks old plants were wounded by clamping a tweezers across the midvein. RNA was extracted from control and systemic leaves 2 h after wounding, and was subject to affymetrix ATH1 chip. one repeat: wt control, wt systemic wounding, opr3 control, opr3 systemic wounding

2010-12-01 | E-GEOD-14332 | biostudies-arrayexpress

wt and opr3 control and systemic wounding

2010-12-01 | GSE14332 | GEO

Identification of MKK3-dependent wounding-induced genes

Project description:ra16-01_mkk3_wounding - identification of mkk3-dependent wounding-induced genes - Is MKK3 involved in the transcriptional regulation of wounding-induced genes? - Col and mkk3 KO plants were wounded with a forceps. Leaves were harvested 2h after the stress.

2017-03-10 | GSE95621 | GEO

Proteomic analysis of oil palm's response to red palm weevil infestation

Project description:Oil palm leaves were analysed via proteomics approach to identify the differentially-expressed proteins under Red Palm Weevil infestation on the first, third and sixth week post-infestation. The comparison was made among three groups; the control (C), physical wounding by drilling (wounded,W) and the red palm weevil larva infestation (Infested, I)

2022-02-14 | PXD028986 | Pride

Current injection and distal wound response in Arabidopsis thaliana

Project description:These data represent two experiments, one on wounding leaves and one based on injecting current into leaves. We first analyzed transcript levels in leaf 13 when leaf 8 was wounded. Transcripts levels in leaf 8 were then analyzed after current injection. Sample handling and normalisations were identical between experiments.

2013-08-21 | GSE41779 | GEO

Small RNAs from sweet potato upon wounding

Project description:Small RNAs (sRNAs) play important roles in plants encountering stress environments. However, limited research has been conducted on the sRNAs involved in plant wound responses. To identify potential roles for the wounding-related sRNAs, sRNA deep sequencing was used. After leaves were wounded for 0.5 hour, total RNAs from unwounded and wounded leaves were isolated for sRNA library construction. The Illumina platform was used to sequence sRNA libraries. About 12 million sequence reads were obtained for each sample.

2016-04-26 | GSE46457 | GEO

Expression data from arabidopsis wild type (Col-0) and JAV1 RNAi plant

Project description:JAV1 functions as a negative regulator to control plant defense. Defense-related genes were highly induced in JAV1 RNAi plants compared to wild-type plants in response to wounding or jasmonate treatments. We used microarrays to detail the global programme of gene expression underlying JAV1 effects on expression of genes involving in defense response, lipid metabolism and regulating transcription. For the Affymetrix microarray assay with 100 uM MeJA treatment, 21-day-old seedlings grown in MS medium were treated with 100 μM MeJA for various time periods (0, 1, and 4 hours). For the Affymetrix microarray assay with wounding treatment, JAV1 RNAi line Ri17 versus wild-type was utilized after mechanical wounding for various time periods. Four rosette leaves from 21-day-old plants were crushed across the midrib with a forceps. About 30% of each leaf was damaged. Wounded leaves were harvested at various time periods (0, 0.5, and 1 hour) for RNA extraction. Control plants were untreated and leaves were harvested for RNA extraction.

2013-04-02 | E-GEOD-45662 | biostudies-arrayexpress

Transcriptome Analysis of Wounding-Induced Root to Shoot Communication in Arabidopsis thaliana.

Project description:Comparison of gene expression between shoots of root-wounded seedlings and shoots of control seedlings in Arabidopsis. We identified wounding-induced early (30 min) and late (360 min) root to shoot responsive genes (RtS).

2010-12-31 | GSE26374 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data