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Brain gene expression in mice mono-colonized with E.coli HA107

ABSTRACT: The gut microbiota can influence brain function and behaviour through several mechanisms. The first critical step that initiates microbial-host interaction which leads to the establishment of normal behavior is unknown. In this study, we identified that activation of the innate immune system by microbes is a critical step in the initiation of the gut-brain communication. We showed that bacterial mono-colonization was sufficient to initiate the normalization of behavior which critically depends on Toll-like receptors. To investigate the mechanisms by which immune system activation affects behavior, we assessed the brain region specific (hippocampus and amygdala) gene expression from germ-free Swiss Webster mice before and after mono-colonization with E.coli HA107 bacterial strain. Gene expression analysis showed that colonization affected multiple pathways related to innate immunity and neuronal function, including canonical pathway of glucocorticoid receptor that play key role in regulating emotion and cognition. Several genes directly involved in neuronal plasticity were altered by colonization, including phenotypic switch in NMDA receptors subunits, which in conventional animals is observed after birth and possibly linked to initial exposure to microbiome. These results raise the possibility that impaired postnatal microbiota-host cross talk could affect normal brain maturation process and lead to altered neurodevelopment and psychiatric disorders later in life.

ORGANISM(S): Mus musculus

PROVIDER: GSE138320 | GEO | 2025/12/01

REPOSITORIES: GEO

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Colonic gene expression in mice mono-colonized with E.coli HA107

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