Project description:A 24h time-course array study was performed to analyse the different circadian phenotypes of LCL-H0 and HD-MY-Z cells, a cell line from B lymphoblastoid cells and a Hodgkin lymphoma cell line. Samples were taken every three hours for a period of 24h so that 9 time-points could be analysed for each cell line.
Project description:Interventions: For mucosal defect after ESD, only conventional clips are used and closed using Clip on Clip Closure Method.
Primary outcome(s): Evaluate whether mucosal defect after ESD is closed by Clip on Clip Closure Method using conventional clip.
Study Design: Single arm Non-randomized
Project description:The identification of RNAs that are recognized by RNA-binding proteins (RNA-BPs) using techniques such as Crosslinking and Immunoprecipitation (CLIP) has revolutionized the genome-wide discovery of RNA-BP RNA targets. Among the different versions of CLIP that have been developed, the use of photoactivable nucleoside analogs has resulted in high efficiency photoactivable ribonucleoside-enhanced CLIP (PAR-CLIP) in vivo. Nonetheless, PAR-CLIP has not yet been applied in prokaryotes. To determine if PAR-CLIP can be used in prokaryotes, we determined suitable conditions for the incorporation of 4-thiouridine (4SU), a photoactivable nucleoside, into E. coli RNA and for the isolation of RNA crosslinked to RNA-BPs of interest. Applying this technique to Hfq, a well-characterized regulator of small RNA (sRNA)-messenger RNA (mRNA) interactions, we showed that PAR-CLIP identified most of the known sRNA targets of Hfq, as well as functionally relevant sites of Hfq-mRNA interactions at nucleotide resolution. Based on our findings, PAR-CLIP represents an improved method to identify both the RNAs and the specific regulatory sites that are recognized by RNA-BPs in prokaryotes.
