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A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE138675 | GEO | 2019/12/08

REPOSITORIES: GEO

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Similar Datasets

A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.

Project description: Not available

| S-EPMC7062732 | biostudies-literature

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

Project description:PRDM14 is a crucial regulator of mouse primordial germ cells (mPGC), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of human PGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.

2019-12-08 | GSE138674 | GEO

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

2019-12-08 | GSE138673 | GEO

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

Project description:A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

| PRJNA576722 | ENA

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

Project description:A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

| PRJNA576723 | ENA

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

Project description:A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

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SOX17 is a critical specifier of human primordial germ cell fate.

Project description: Not available

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Testing the role of SOX15 in human primordial germ cell fate.

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Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.

Project description: Not available

| S-EPMC4406649 | biostudies-literature

SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate

Project description:Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state. However, without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. Here, we demonstrate specification of hPGC-like cells (hPGCLCs) from germline competent pluripotent stem cells. The characteristics of hPGCLCs are consistent with the embryonic hPGCs and a germline seminoma that share a CD38 cell-surface marker, which collectively defines likely progression of the early human germline. Remarkably, SOX17 is the key regulator of hPGC-like fate, whereas BLIMP1 represses endodermal and other somatic genes during specification of hPGCLCs. Notable mechanistic differences between mouse and human PGC specification could be attributed to their divergent embryonic development and pluripotent states, which might affect other early cell-fate decisions. We have established a foundation for future studies on resetting of the epigenome in hPGCLCs and hPGCs for totipotency and the transmission of genetic and epigenetic information. RNA-Seq analysis to investigate transcriptomes of hPGC-like cells (hPGCLCs), fetal hPGCs, TCam-2 and hESCs

2014-12-26 | E-GEOD-60138 | biostudies-arrayexpress

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