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Genome-wide analysis upon ZBTB18 expression and/or CTBP2 silencing in Glioblastoma

ABSTRACT: We have identified the CTBP2 protein as a new ZBTB18 interactor. Here we have evaluated transcriptomic changes upon CTBP2 knockdown in the GBM cell line SNB19. Moreover, we have analyzed whether CTBP2 is required for ZBTB18-mediated gene repression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE138890 | GEO | 2019/12/01

REPOSITORIES: GEO

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Genome wide mapping of binding sites of ZBTB18 and CTBP2 in glioblastoma cancer cells

Project description:CTBP2 is a transcriptional co-repressor/co-activator palying a role in EMT. We have identified CTBP2 as a protein interacting with ZBTB18, a transcriptional repressor and tumor suppressor in GBM. Here, we have performed genome wide mapping of CTBP2 and ZBTB18 binding sites in GBM to characterized their gene regulation mechanism.

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Genome-wide analysis upon ZBTB18 expression and/or CTBP2 silencing in Glioblastoma

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Analysis of gene expression changes upon ZBTB18 full length (FL), ZBTB18 full length-mutant (FL-mut), ZBTB18 short form Nte (SF-Nte) and ZBTB18 short form Nte (SF-mut) in SNB19 glioblastoma cell line

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Analysis of gene expression changes upon ZBTB18 full length (FL), ZBTB18 full length-mutant (FL-mut), ZBTB18 short form Nte (SF-Nte) and ZBTB18 short form Nte (SF-mut) in SNB19 glioblastoma cell line

Project description:Analysis of gene expression changes upon ZBTB18 full length (FL), ZBTB18 full length-mutant (FL-mut), ZBTB18 short form Nte (SF-Nte) and ZBTB18 short form Nte (SF-mut) in SNB19 glioblastoma cell line

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ZBTB18 is a repressor of mesenchymal genes in Glioblastoma [JX6]

Project description:The transcriptional repressor ZBTB18 was overexpressed in the brain tumor xenoline JX6 by lentiviral transduction. Three independent transduction were performed (biological replicates) and analyzed by gene expression aray. Gene set enrichemnt analysis (GSEA) showed changes in the expression of mesenchymal signature. A subset of genes was further valiadted by qPCR. These results indicate a role of ZBTB18 as repressor of mesenchymal genes in Glioblastoma.

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ZBTB18 is a repressor of mesenchymal genes in Glioblastoma [BTSC233]

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Effect of ZBTB18 on gene expression and chromatin organization [RNA-seq]

Project description:This study set out to delineate gene expression programs that are differentially modulated in metastatic breast cancer cells with high versus low ability to further metastasize. To this end, the gene expression profiles of E0771GFP cells, M11GFP and M12GFP cells were determined. Analysis of these data sets identified ZBTB18 as a transcriptional repressor with lower activity in tumor cells that retain a high metastatic potential. To determine how ZBTB18 affects gene expression and chromatin organization, ZBTB18 was overexpressed in E0771GFP breast cancer cells. Gene expression profiles and differentially accessible DNA regions analyses were performed and identified Tgfbr2 as one of the genes most significantly repressed by ZBTB18. To determine the contribution of the TGFb1 -TGFBR2 signaling axis towards ZBTB18-mediated effects, E0771GFP cells overexpressing ZBTB18 were transduced with a doxycycline-inducible Tgfbr2 construct. These cells were then treated with doxycycline and recombinant TGFb1 or vehicle control and gene expression profiles were determined. These studies revealed that inhibition of ZBTB18 activity in tumor cells promotes metastasis by increasing chromatin accessibility and by enabling pro-metastatic TGFb1-TGFBR2-driven changes.

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Effect of ZBTB18 on gene expression and chromatin organization [ATAC-seq]

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The chromatin landscape modified by Zbtb18 in activated B cells

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2020-09-01 | GSE129657 | GEO

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